EN ISO 20743:2007
(Main)Textiles - Determination of antibacterial activity of antibacterial finished products (ISO 20743:2007)
Textiles - Determination of antibacterial activity of antibacterial finished products (ISO 20743:2007)
ISO 20743:2007 specifies quantitative test methods to determine the antibacterial activity of antibacterial finished textile products including nonwovens.
ISO 20743:2007 is applicable to all textile products, including cloth, wadding, thread and material for clothing, home furnishings and miscellaneous goods regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).
Based on the intended application and on the environment in which the textile product is to be used, the user can select the most suitable of the following three methods on determination of antibacterial activity: a) absorption method (an evaluation method in which test bacterial suspension is inoculated directly onto samples); b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto samples); c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto samples).
The colony plate count method and the ATP (Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.
Textilien - Bestimmung der antibakteriellen Wirkung antibakteriell behandelter Erzeugnisse (ISO 20743:2007)
Die vorliegende Internationale Norm legt quantitative Prüfverfahren zur Bestimmung der antibakteriellen Wirkung antibakteriell behandelter textiler Erzeugnisse, einschließlich Vliesstoffe fest.
Diese Internationale Norm ist anwendbar auf alle textilen Erzeugnisse, einschließlich Stoffe, Wattierung, Garn und Materialien für Bekleidung, Heimtextilien sowie verschiedene Waren, ungeachtet des Typs des verwendeten antibakteriellen Mittels (organisch, anorganisch, natürlich oder künstlich) oder des Anwendungs¬verfahrens (eingebaut, Nachbehandlung oder Pfropfen).
Auf der Grundlage der beabsichtigten Anwendung und der Umgebung, für die das textile Erzeugnis zu verwenden ist, kann der Anwender aus den folgenden drei Verfahren zur Bestimmung der antibakteriellen Wirkung das geeignetste auswählen.
a) Absorptionsverfahren (ein Bewertungsverfahren, bei dem die bakterielle Prüfsuspension direkt auf Proben geimpft wird);
b) Übertragungsverfahren (ein Bewertungsverfahren, bei dem Prüfbakterien auf eine Agarplatte aufgebracht und auf Proben übertragen werden);
c) Stempelverfahren (ein Bewertungsverfahren, bei dem Prüfbakterien auf ein Filter aufgebracht und auf Proben gestempelt werden).
Das Kolonie-Plattenzählverfahren und das ATP-Lumineszenzverfahren (ATP = Adenosintriphosphat) werden auch zum Messen der Bakterienzahl festgelegt.
Textiles - Détermination de l'activité antibactérienne des produits finis antibactériens (ISO 20743:2007)
L'ISO 20743:2007 spécifie des méthodes d'essai quantitatives permettant de déterminer l'activité antibactérienne des produits textiles finis antibactériens, y compris les non-tissés.
L'ISO 20743:2007 s'applique à tous les produits textiles, y compris le tissu, le rembourrage, le fil et les matériaux utilisés dans la confection de vêtements, de tissus d'ameublements et d'articles divers, quel que soit le type d'agent antibactérien utilisé (organique, inorganique, naturel ou synthétique) ou quelle que soit la méthode d'application (intégration, post-traitement ou greffage).
Tenant compte de l'application prévue et de l'environnement dans lequel le produit textile est destiné à être utilisé, l'utilisateur peut choisir la plus adaptée des trois méthodes suivantes de détermination de l'activité antibactérienne: a) méthode par absorption (méthode d'évaluation dans laquelle la suspension bactérienne à l'essai est ensemencée directement sur des échantillons); b) méthode par transfert (méthode d'évaluation dans laquelle les bactéries soumises à l'essai sont placées sur une boîte de milieu gélosé, puis transférées sur des échantillons); c) méthode par impression (méthode d'évaluation dans laquelle les bactéries soumises à l'essai sont placées sur un filtre, puis imprimées sur des échantillons);
La technique de dénombrement et la méthode de mesure de luminescence ATP (triphosphate d'adénosine) sont également spécifiées pour le dénombrement des bactéries.
Tekstilije - Ugotavljanje protibakterijske aktivnosti na izdelkih s protibakterijsko apreturo (ISO 20743:2007)
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-julij-2007
Tekstilije - Ugotavljanje protibakterijske aktivnosti na izdelkih s protibakterijsko
apreturo (ISO 20743:2007)
Textiles - Determination of antibacterial activity of antibacterial finished products (ISO
20743:2007)
Textilien - Bestimmung der antibakteriellen Wirkung antibakteriell behandelter
Erzeugnisse (ISO 20743:2007)
Textiles - Détermination de l'activité antibactérienne des produits finis antibactériens
(ISO 20743:2007)
Ta slovenski standard je istoveten z: EN ISO 20743:2007
ICS:
59.080.01 Tekstilije na splošno Textiles in general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 20743
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2007
ICS 59.080.01; 07.100.99
English Version
Textiles - Determination of antibacterial activity of antibacterial
finished products (ISO 20743:2007)
Textiles - Détermination de l'activité antibactérienne des Textilien - Bestimmung der antibakteriellen Wirkung
produits finis antibactériens (ISO 20743:2007) antibakteriell behandelter Erzeugnisse (ISO 20743:2007)
This European Standard was approved by CEN on 16 May 2007.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 20743:2007: E
worldwide for CEN national Members.
Foreword
This document (prEN ISO 20743:2007) has been prepared by Technical Committee ISO/TC 38
"Textiles" in collaboration with Technical Committee CEN/TC 248 "Textiles and textile products",
the secretariat of which is held by BSI.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by December 2007, and conflicting national
standards shall be withdrawn at the latest by December 2007.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United
Kingdom.
Endorsement notice
The text of ISO 20743:2007 has been approved by CEN as EN ISO 20743:2007 without any
modifications.
INTERNATIONAL ISO
STANDARD 20743
First edition
2007-06-01
Textiles — Determination of antibacterial
activity of antibacterial finished products
Textiles — Détermination de l'activité antibactérienne des produits finis
antibactériens
Reference number
ISO 20743:2007(E)
©
ISO 2007
ISO 20743:2007(E)
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ii © ISO 2007 – All rights reserved
ISO 20743:2007(E)
Contents Page
Foreword. iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions. 1
4 Safety precaution. 2
5 Apparatus . 2
6 Reagents and culture media. 4
7 Reference strains. 8
7.1 Strains. 8
7.2 Storage of strains . 8
8 Quantitative measurement. 10
8.1 Plate count method. 10
8.2 Luminescence method. 11
9 Shaking method. 12
9.1 Shaking by vortex mixer . 12
9.2 Shaking by hand . 12
9.3 Shaking by Stomacher . 12
10 Test procedures . 12
10.1 Absorption method. 12
10.2 Transfer method. 17
10.3 Printing method . 21
Bibliography . 27
ISO 20743:2007(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 20743 was prepared by Technical Committee ISO/TC 38, Textiles.
iv © ISO 2007 – All rights reserved
ISO 20743:2007(E)
Introduction
These test methods were established in order to address the substantial need for an International Standard to
determine antibacterial activity for antibacterial finished textile products.
INTERNATIONAL STANDARD ISO 20743:2007(E)
Textiles — Determination of antibacterial activity of
antibacterial finished products
1 Scope
This International Standard specifies quantitative test methods to determine the antibacterial activity of
antibacterial finished textile products including nonwovens.
This International Standard is applicable to all textile products, including cloth, wadding, thread and material
for clothing, home furnishings and miscellaneous goods regardless of the type of antibacterial agent used
(organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).
Based on the intended application and on the environment in which the textile product is to be used, the user
can select the most suitable of the following three methods on determination of antibacterial activity:
a) absorption method (an evaluation method in which test bacterial suspension is inoculated directly onto
samples);
b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred
onto samples);
c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto
samples).
The colony plate count method and the ATP (ATP = Adenosine Tri-phosphate) luminescence method are
also specified for measuring the enumeration of bacteria.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 6330, Textiles — Domestic washing and drying procedures for textile testing
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
textile
fabric
general term employed for designating textile surfaces, woven fabrics, knitted fabrics, etc., formed by the
interlocking of textile materials having a certain cohesion and which are generally intended for clothing or
furniture applications
NOTE Often includes certain types of nonwovens.
ISO 20743:2007(E)
3.2
control fabric
fabric used to validate the growth condition of test bacteria
NOTE The same fabric as the fabric to be tested but without antibacterial treatment. If this is not possible, then
100 % cotton fabric without fluorescent brighteners or other finish.
3.3
antibacterial agent
product designed to prevent or mitigate the growth of bacteria, to reduce the number of bacteria or to kill
bacteria
3.4
antibacterial finish
treatment designed to prevent or mitigate the growth of bacteria, to reduce the number of bacteria or to kill
bacteria
3.5
antibacterial activity
activity of an antibacterial finish used to prevent or mitigate the growth of bacteria, to reduce the number of
bacteria or to kill bacteria
3.6
plate count method
method in which the number of bacteria present after incubation is calculated by counting the number of
colonies according to a ten-time dilution method
NOTE The results are expressed in “CFU (Colony Forming Unit)”.
3.7
luminescence method
method in which the amount of ATP contained in bacterial cells is measured
NOTE The results are expressed in “mol of ATP”.
3.8
neutralizer
chemical agents used to inactivate, neutralize, or quench the antibacterial properties of antibacterial agents
4 Safety precaution
Test methods specified herein require the use of bacteria.
These tests should be carried out by persons with training and experience in the use of microbiological
techniques.
Appropriate safety precautions should be observed with
...
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