EN ISO 17678:2010

Milk and milk products - Determination of milk fat purity by gas chromatographic analysis of triglycerides (Reference method) (ISO 17678:2010)

ISO 17678|IDF 202:2010 specifies a reference method for the determination of milk fat purity using gas chromatographic analysis of triglycerides. Both vegetable fats and animal fats such as beef tallow and lard can be detected. By using defined triglyceride equations, the integrity of milk fat is determined.
Basically, the method applies to bulk milk, or products made thereof, irrespective of feeding, breed or lactation conditions. In particular, the method is applicable to fat extracted from milk products purporting to contain pure milk fat with unchanged composition, such as butter, cream, milk, and milk powder.
However, under the circumstances listed hereafter, a false positive result can be obtained. Hence, the method is not applicable to milk fat: a) obtained from bovine milk other than cow's milk; b) obtained from single cows; c) obtained from cows which received an exceptionally high feeding of pure vegetable oils such as rapeseed oil; d) obtained from colostrum; e) subjected to technological treatment such as removal of cholesterol or fractionation; f) obtained from skim milk or buttermilk; g) extracted by using the Gerber, Weibull–Berntrop or Schmid–Bondzynski–Ratzlaff methods, or that has been isolated using detergents (e.g. the Bureau of Dairy Industries method).
With the extraction methods specified in g), substantial quantities of partial glycerides or phospholipids can pass into the fat phase. Consequently, the scope of ISO 17678|IDF 202:2010 excludes certain products and particularly cheese, whose ripening process can also affect the fat composition to such a degree that a false positive result is obtained.

Milch und Milcherzeugnisse - Bestimmung der Reinheit des Milchfetts durch gaschromatographische Triglyceridanalyse (Referenzverfahren) (ISO 17678:2010)

Diese Internationale Norm legt ein Referenzverfahren zur Bestimmung der Reinheit von Milchfett durch gaschromatographische Triglyceridanalyse fest. Es können sowohl pflanzliche als auch tierische Fette wie Rindertalg und Schweinefett nachgewiesen werden. Unter Verwendung festgelegter Triglyceridgleichungen wird die Integrität des Milchfetts bestimmt.
Grundsätzlich gilt das Verfahren für Sammelmilch oder daraus hergestellte Produkte, unabhängig von der Rasse sowie den Fütterungs  oder Laktationsbedingungen. Insbesondere ist das Verfahren auf Fett anwendbar, das aus Milchprodukten extrahiert wurde, die reines Milchfett in unveränderter Zusammensetzung enthalten sollen, wie z. B. Butter, Sahne, Milch und Milchpulver.
Jedoch kann in den nachstehend erwähnten Fällen ein falsch positives Ergebnis ermittelt werden. Deshalb ist das Verfahren nicht anwendbar auf Milchfett,
a)   das aus anderer Milch als Kuhmilch gewonnen wurde;
b)   das von einzelnen Kühen gewonnen wurde;
c)   das von Kühen gewonnen wurde, die eine außergewöhnlich hohe Fütterung von reinen Pflanzenölen wie Rapsöl erhalten haben;
d)   das aus Kolostrum (Vormilch) gewonnen wurde;
e)   das einer technologischen Behandlung unterzogen wurde, wie z. B. dem Entfernen von Cholesterol oder einer Fraktionierung;
f)   das aus Magermilch oder Buttermilch gewonnen wurde;
g)   das mit Verfahren nach Gerber, Weibull Berntrop oder Schmid Bondzynski Ratzlaff extrahiert wurde oder das mittels Detergenzien isoliert wurde [z. B. Bureau of Dairy Industries (BDI)-Verfahren].
Mit den in g) festgelegten Extraktionsverfahren können beträchtliche Mengen von Partialglyceriden oder Phospholipiden in die Fettphase übertreten. Folglich werden bestimmte Produkte und insbesondere Käse, dessen Reifungsprozess die Fettzusammen¬setzung zusätzlich derartig beeinflussen kann, dass ein falsch-positives Ergebnis erhalten wird, aus dem Anwendungsbereich dieser Internationalen Norm ausgeschlossen.
(...)

Lait et produits laitiers - Détermination de la pureté des matières grasses laitières par analyse chromatographique en phase gazeuse des triglycérides (Méthode de référence) (ISO 17678:2010)

L'ISO 17678|FIL 202:2010 spécifie une méthode de référence pour la détermination de la pureté des matières grasses laitières par analyse chromatographique en phase gazeuse des triglycérides. La méthode permet de détecter des matières grasses d'origines végétale et animale telles que la graisse de bœuf et le saindoux. À l'aide de formules de triglycérides définies, l'intégrité de la matière grasse laitière est ainsi déterminée.
En principe, la méthode s'applique au lait en vrac, ou aux produits laitiers dérivés, indépendamment des conditions d'alimentation, d'élevage ou de lactation. Elle s'applique en particulier aux matières grasses extraites de produits laitiers supposés contenir des matières grasses laitières pures, présentant une composition non modifiée, comme le beurre, la crème, le lait et le lait en poudre.
Toutefois, dans les circonstances listées ci-après, un résultat faux positif peut être obtenu. Par conséquent, la méthode n'est pas applicable à la matière grasse laitière: a) issue de lait de bovin autre que le lait de vache; b) issue de vaches individuelles; c) issue de vaches ayant reçu une alimentation exceptionnellement riche en huiles végétales pures telles que l'huile de colza; d) issue du colostrum; e) soumise à un traitement technologique tel que l'élimination du cholestérol ou le fractionnement; f)issue du lait écrémé ou du lait battu (babeurre); g) extraite selon les méthodes Gerber, Weibull-Berntrop ou Schmid-Bondzynski-Ratzlaff, ou isolée au moyen de détergents [par exemple méthode BDI (Bureau of Dairy Industries)].
Avec les méthodes d'extraction spécifiées en g), d'importantes quantités de glycérides ou phospholipides partiaux peuvent passer dans la phase des matières grasses. Par conséquent, le domaine d'application de l'ISO 17678|FIL 202:2010 exclut certains produits et en particulier le fromage, dont le procédé d'affinage peut également affecter la composition de la matière grasse à tel point qu'un résultat faux positif est obtenu.

Mleko in mlečni proizvodi - Določevanje čistosti mlečne maščobe s plinsko kromatografsko analizo trigliceridov (referenčna metoda) (ISO 17678:2010)

Ta mednarodni standard določa referenčno metodo za določevanje čistosti mlečnih maščob s plinsko kromatografsko analizo trigliceridov. Tako rastlinske kot živalske maščobe kot goveji loj in svinjska mast so lahko zaznane. Z uporabo definiranih trigliceridnih enačb je določena celotna mlečna maščoba.  V bistvu metoda velja za večje količine mleka ali proizvode, izdelane iz njega, ne glede na krmljenje, pasmo ali pogoje izločanja mleka. Zlasti metoda velja za maščobe, ekstrahirane iz mlečnih proizvodov, ki domnevno vsebujejo čisto mlečno maščobo z nespremenjeno sestavo, kot so maslo, smetana, mleko in mleko v prahu. Vendar pod okoliščinami naštetimi v nadaljevanju, lahko dosežemo lažen pozitiven rezultat. Zato metoda ne velja za mlečne maščobe:
a) pridobljene iz govejega mleka razen kravjega mleka;
b) pridobljene iz posameznih krav;
c) pridobljene iz krav, ki so dobile izjemno veliko krme iz čistih rastlinskih olj, kot je na primer olje iz repičnih semen;
d) pridobljene iz kolostruma;
d) izpostavljene tehnološkim obdelavam, kot so odstranitev holesterola ali frakcioniranje;
f) pridobljene iz posnetega mleka ali sirotke;
g) ekstrahirane z uporabo Gerber, Weibull-Berntrop ali Schmid-Bondzynski-Ratzlaff metod, ali pa so bile izolirane z uporabo čistil (npr. metoda Urada za mlečno industrijo). Z metodami ekstrahiranja določenimi v g) lahko precejšne količine delnih gliceridov ali fosfolipidov preide v maščobno fazo. Posledično obseg tega mednarodnega standarda izključuje določene proizvode in posebne sire, katerih proces zorenja lahko vpliva na sestavo maščob do te mere, da lahko dobimo lažen pozitiven rezultat. OPOMBA 1: V naravi se butanojska (n-butanojska) kislina (C4) pojavlja samo v mlečni maščobi in omogoča izdelavo kvantitativne ocene nizkih do srednjih vrednostih mlečne maščobe v rastlinskih in živalskih maščobah. Vendar je zaradi velikih odstopanj C4, katerega približna vrednost je od 3.1 % masne frakcije do 3.8 masne frakcije, težko zagotoviti kvalitativne in kvantitativne informacije za razmerje tuje maščobe in čiste mlečne maščobe tudi do 20 % masne frakcije (glej Referenco [11]). OPOMBA: 2 V praksi se kvantitativni rezultati ne morejo izpeljati iz vsebnosti sterola v rastlinskih maščobah zaradi odvisnosti od pogojev pridelave in obdelave. Nadalje, kvalitativno določevanje tuje maščobe z uporabo sterolov je dvoumno.

General Information

• Status: Withdrawn
• Publication Date: 14-Feb-2010
• Withdrawal Date: 25-Jun-2019
• ICS: 67.100.99 - Other milk products
• ICS: 67.200.10 - Animal and vegetable fats and oils
• Technical Committee: CEN/TC 302 - Milk and milk products - Methods of sampling and analysis
• Drafting Committee: ISO/TC 34 - ISO/TC 34
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Completion Date: 26-Jun-2019
• Ref Project: SIST EN ISO 17678:2010 - Milk and milk products - Determination of milk fat purity by gas chromatographic analysis of triglycerides (Reference method) (ISO 17678:2010)

Relations

• Replaced By: EN ISO 17678:2019 - Milk and milk products - Determination of milk fat purity by gas chromatographic analysis of triglycerides (ISO 17678:2019)
• Effective Date: 03-Jul-2019

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2010
0OHNRLQPOHþQLSURL]YRGL'RORþHYDQMHþLVWRVWLPOHþQHPDãþREHVSOLQVNR
NURPDWRJUDIVNRDQDOL]RWULJOLFHULGRYUHIHUHQþQDPHWRGD,62
Milk and milk products - Determination of milk fat purity by gas chromatographic analysis
of triglycerides (Reference method) (ISO 17678:2010)
Milch und Milcherzeugnisse - Bestimmung der Reinheit des Milchfetts durch
gaschromatographische Triglyceridanalyse (Referenzverfahren) (ISO 17678:2010)
Lait et produits laitiers - Détermination de la pureté des matières grasses du lait par
analyse chromatographique en phase gazeuse des triglycérides (Méthode de référence)
(ISO 17678:2010)
Ta slovenski standard je istoveten z: EN ISO 17678:2010
ICS:
67.100.10 0OHNRLQSUHGHODQLPOHþQL Milk and processed milk
SURL]YRGL products
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 17678
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2010
ICS 67.100.99; 67.200.10
English Version
Milk and milk products - Determination of milk fat purity by gas
chromatographic analysis of triglycerides (Reference method)
(ISO 17678:2010)
Lait et produits laitiers - Détermination de la pureté des Milch und Milcherzeugnisse - Bestimmung der Reinheit des
matières grasses laitières par analyse chromatographique Milchfetts durch gaschromatographische Triglyceridanalyse
en phase gazeuse des triglycérides (Méthode de référence) (Referenzverfahren) (ISO 17678:2010)
(ISO 17678:2010)
This European Standard was approved by CEN on 13 February 2010.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.

EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2010 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 17678:2010: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
This document (EN ISO 17678:2010) has been prepared by Technical Committee ISO/TC 34 "Food products"
in collaboration with Technical Committee CEN/TC 302 “Milk and milk products - Methods of sampling and
analysis”, the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by August 2010, and conflicting national standards shall be withdrawn at
the latest by August 2010.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 17678:2010 has been approved by CEN as an EN ISO 17678:2010 without any modification.

INTERNATIONAL ISO
STANDARD 17678
IDF
First edition
2010-02-15
Milk and milk products — Determination
of milk fat purity by gas chromatographic
analysis of triglycerides (Reference
method)
Lait et produits laitiers — Détermination de la pureté des matières
grasses laitières par analyse chromatographique en phase gazeuse des
triglycérides (Méthode de référence)

Reference numbers
ISO 17678:2010(E)
IDF 202:2010(E)
©
ISO and IDF 2010
ISO 17678:2010(E)
IDF 202:2010(E)
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Published in Switzerland
ii © ISO and IDF 2010 – All rights reserved

ISO 17678:2010(E)
IDF 202:2010(E)
Contents Page
Foreword .iv
1 Scope.1
2 Normative references.2
3 Terms and definitions .2
4 Principle .2
5 Reagents .2
6 Apparatus.3
7 Sampling .4
8 Procedure.4
8.1 Preparation of test samples .4
8.2 Preparation of fat sample solution .5
8.3 Chromatographic triglyceride determination .5
9 Integration, evaluation and control of the analytical performance .8
10 Calculation and expression of results .9
10.1 Triglyceride composition.9
10.2 S-values .10
10.3 Detection of foreign fat .10
11 Precision .11
11.1 Interlaboratory test.11
11.2 Repeatability .11
11.3 Reproducibility .12
12 Test report.12
Annex A (normative) Preparation of the packed column .13
Annex B (informative) Quantification of the foreign fat content.17
Annex C (informative) Uncertainty of measurement .19
Annex D (informative) Interlaboratory test.20
Bibliography.22

ISO 17678:2010(E)
IDF 202:2010(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has
been established has the right to be represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 17678⎪IDF 202 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 5,
Milk and milk products, and the International Dairy Federation (IDF). It is being published jointly by ISO and
IDF.
iv © ISO and IDF 2010 – All rights reserved

ISO 17678:2010(E)
IDF 202:2010(E)
Foreword
IDF (the International Dairy Federation) is a non-profit organization representing the dairy sector worldwide.
IDF membership comprises National Committees in every member country as well as regional dairy
associations having signed a formal agreement on cooperation with IDF. All members of IDF have the right to
be represented on the IDF Standing Committees carrying out the technical work. IDF collaborates with ISO in
the development of standard methods of analysis and sampling for milk and milk products.
The main task of Standing Committees is to prepare International Standards. Draft International Standards
adopted by the Standing Committees are circulated to the National Committees for endorsement prior to
publication as an International Standard. Publication as an International Standard requires approval by at least
50 % of the IDF National Committees casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. IDF shall not be held responsible for identifying any or all such patent rights.
ISO 17678⎪IDF 202 was prepared by the International Dairy Federation (IDF) and Technical Committee
ISO/TC 34, Food products, Subcommittee SC 5, Milk and milk products. It is being published jointly by IDF
and ISO.
All work was carried out by the Joint ISO-IDF Project Group on Foreign fats of the Standing Committee on
Analytical methods for composition under the aegis of its project leader, Dr J. Molkent
...