SIST EN 12014-2:2018

Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for the determination of nitrate content of vegetables and vegetable products

This European Standard specifies a high-performance liquid chromatographic (HPLC) and an ion chromatographic (IC) method for determination of the nitrate level in vegetables and vegetable products. This method is applicable for samples with a content of 25 mg/kg or greater.
It has been validated on naturally contaminated and spiked samples as beetroot juice with nitrate mass fractions of 194 mg/kg and 691 mg/kg, pureed carrots with nitrate mass fractions of 26 mg/kg and 222 mg/kg and with iceberg lettuce with nitrate mass fractions of 623 mg/kg and 3 542 mg/kg.

Lebensmittel - Bestimmung des Nitrat- und/oder Nitritgehaltes - Teil 2: HPLC/IC-Verfahren für die Bestimmung des Nitratgehaltes in Gemüse und Gemüseerzeugnissen

Diese Europäische Norm legt ein hochleistungs-flüssigchromatographisches (HPLC) und ein ionenchromatographisches (IC) Verfahren zur Bestimmung des Nitratgehaltes in Gemüse und Gemüseerzeugnissen fest. Dieses Verfahren ist auf Proben mit einem Gehalt von 25 mg/kg oder größer anwendbar.
Es wurde mit natürlich kontaminierten und aufgestockten Proben in Rote-Beete-Saft mit Nitrat-Massenanteilen von 194 mg/kg und 691 mg/kg, auf pürierte Karotten mit Nitrat-Massenanteilen von 26 mg/kg und 222 mg/kg sowie auf Eisbergsalat mit Nitrat-Massenanteilen von 623 mg/kg und 3 542 mg/kg validiert.

Produits alimentaires - Détermination de la teneur en nitrates et/ou en nitrites - Partie 2: Méthode de détermination par CLHP/CI de la teneur en nitrates des légumes et des produits à base de légumes

La présente Norme européenne spécifie une méthode de chromatographie en phase liquide à haute performance (CLHP) et une méthode de chromatographie par échange d'ions (Cl) pour déterminer la teneur en nitrates des légumes et des produits à base de légumes. Ces méthodes sont applicables aux échantillons dont la teneur est supérieure ou égale à 25 mg/kg.
Elles ont été validées sur des échantillons dopés et sur des échantillons contaminés naturellement de jus de betterave ayant une teneur en nitrates (fraction massique) de 194 mg/kg et 691 mg/kg, de purée de carottes ayant une teneur en nitrates (fraction massique) de 26 mg/kg et 222 mg/kg, et de laitue iceberg ayant une teneur en nitrates (fraction massique) de 623 mg/kg et 3 542 mg/kg.

Živila - Določevanje vsebnosti nitratov in/ali nitritov - 2. del: Metoda HPLC/IC za določevanje vsebnosti nitratov v zelenjavi in zelenjavnih proizvodih

Ta evropski standard določa metodo tekočinske kromatografije visoke ločljivosti (HPLC) in ionsko kromatografsko metodo (IC) za določevanje ravni nitrata v zelenjavi in zelenjavnih proizvodih. Ta metoda se uporablja za vzorce z vsebnostjo 25 mg/kg ali več.
Metoda je bila potrjena na naravno kontaminiranih in primešanih vzorcih, na primer soku rdeče pese z masnimi deleži nitrata 194 mg/kg in 691 mg/kg, korenjevim pirejem z masnimi deleži nitrata 26 mg/kg in 222 mg/kg ter ledenki z masnimi deleži nitrata 623 mg/kg in 3542 mg/kg.

General Information

• Status: Published
• Public Enquiry End Date: 19-Sep-2016
• Publication Date: 18-Jan-2018
• ICS: 67.050 - General methods of tests and analysis for food products
• ICS: 67.080.20 - Vegetables and derived products
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 14-Dec-2017
• Due Date: 18-Feb-2018
• Completion Date: 19-Jan-2018
• Directive: 882/2004 - Regulation (EC) No 882/2004 of the European Parliament and of the Council of 29 April 2004 onofficial controls performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules
• Ref Project: EN 12014-2:2017 - Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for the determination of nitrate content of vegetables and vegetable products

Relations

• Replaces: SIST EN 12014-2:1999 - Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for the determination of nitrate content of vegetables and vegetable products
• Effective Date: 08-Jun-2022
• Replaces: SIST EN 12014-2:1999 - Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for the determination of nitrate content of vegetables and vegetable products
• Effective Date: 01-Feb-2018

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN 12014-2:2018
01-februar-2018
1DGRPHãþD
SIST EN 12014-2:1999
äLYLOD'RORþHYDQMHYVHEQRVWLQLWUDWRYLQDOLQLWULWRYGHO0HWRGD+3/&,&]D
GRORþHYDQMHYVHEQRVWLQLWUDWRYY]HOHQMDYLLQ]HOHQMDYQLKSURL]YRGLK
Foodstuffs - Determination of nitrate and/or nitrite content - Part 2: HPLC/IC method for
the determination of nitrate content of vegetables and vegetable products
Lebensmittel - Bestimmung des Nitrat- und/oder Nitritgehaltes - Teil 2: HPLC/IC-
Verfahren für die Bestimmung des Nitratgehaltes in Gemüse und Gemüseerzeugnissen
Produits alimentaires - Détermination de la teneur en nitrates et/ou en nitrites - Partie 2:
Méthode de détermination par CLHP/CI de la teneur en nitrates des légumes et des
produits à base de légumes
Ta slovenski standard je istoveten z: EN 12014-2:2017
ICS:
67.050 Splošne preskusne in General methods of tests and
analizne metode za živilske analysis for food products
proizvode
67.080.20 Zelenjava in zelenjavni Vegetables and derived
proizvodi products
SIST EN 12014-2:2018 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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SIST EN 12014-2:2018

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SIST EN 12014-2:2018


EN 12014-2
EUROPEAN STANDARD

NORME EUROPÉENNE

December 2017
EUROPÄISCHE NORM
ICS 67.080.20 Supersedes EN 12014-2:1997
English Version

Foodstuffs - Determination of nitrate and/or nitrite
content - Part 2: HPLC/IC method for the determination of
nitrate content of vegetables and vegetable products
Produits alimentaires - Détermination de la teneur en Lebensmittel - Bestimmung des Nitrat- und/oder
nitrates et/ou en nitrites - Partie 2: Méthode de Nitritgehaltes - Teil 2: HPLC/IC-Verfahren für die
détermination par CLHP/CI de la teneur en nitrates des Bestimmung des Nitratgehaltes in Gemüse und
légumes et des produits à base de légumes Gemüseerzeugnissen
This European Standard was approved by CEN on 15 October 2017.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland,
Turkey and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2017 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 12014-2:2017 E
worldwide for CEN national Members.

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SIST EN 12014-2:2018
EN 12014-2:2017 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Principle . 5
4 Reagents . 5
5 Apparatus and equipment . 6
6 Procedure. 7
6.1 Sample preparation .
...