SIST EN ISO 4833-2:2013/A1:2022

Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 °C by the surface plating technique - Amendment 1: Clarification of scope (ISO 4833-2:2013/Amd 1:2022)

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zur Zählung von Mikroorganismen - Teil 2: Koloniezählung bei 30 °C mittels Oberflächenverfahren - Änderung 1: Klarstellung des Anwendungsbereichs (ISO 4833-2:2013/Amd 1:2022)

[Not available]

Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des micro-organismes - Partie 2: Comptage des colonies à 30 °C par la technique d'ensemencement en surface - Amendement 1: Clarification du domaine d'application (ISO 4833-2:2013/Amd 1:2022)

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje števila mikroorganizmov - 2. del: Štetje kolonij pri 30 °C s tehniko nasajanja na površino - Dopolnilo A1: Razlaga področja uporabe (ISO 4833-2:2013/Amd 1:2022)

General Information

• Status: Published
• Public Enquiry End Date: 19-Sep-2020
• Publication Date: 06-Mar-2022
• ICS: 07.100.30 - Food microbiology
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: KŽP - Agricultural food products
• Current Stage: 6060 - National Implementation/Publication (Adopted Project)
• Start Date: 03-Mar-2022
• Due Date: 08-May-2022
• Completion Date: 07-Mar-2022
• Ref Project: EN ISO 4833-2:2013/A1:2022 - Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 °C by the surface plating technique - Amendment 1: Clarification of scope (ISO 4833-2:2013/Amd 1:2022)

Relations

• Amends: SIST EN ISO 4833-2:2013 - Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating technique (ISO 4833-2:2013)
• Effective Date: 01-Apr-2022

Overview

EN ISO 4833-2:2013/A1:2022 (CEN endorsement of ISO 4833-2:2013/Amd 1:2022) is an amendment that clarifies the scope of the horizontal method for the enumeration of microorganisms by colony count at 30 °C using the surface plating technique. The standard specifies a horizontal, aerobic surface-plating method for microorganisms able to form colonies on solid media after incubation at 30 °C and confirms intended application across the food chain.

Key topics and technical requirements

• Method type: Horizontal method - colony count by surface plating (manual spread plating) and includes the use of an automated spiral plater (see Annex A) for surface colony counts.
• Incubation conditions: Aerobic incubation at 30 °C.
• Organisms covered: Microorganisms able to grow and form colonies on the surface of solid medium under the specified conditions.
• Minimum count guideline: Technique is suitable for samples that yield a minimum of 10 colonies per plate, corresponding broadly to expected levels of >100 cfu/ml for liquid samples or >1 000 cfu/g for solid samples.
• Scope of application: Applies to products intended for human consumption, products for animal feeding (including pets), environmental samples from food and feed production/handling areas, and all samples from primary production.
• Suitability notes and limitations: Especially suited to samples with heat‑sensitive organisms (e.g., psychrotrophs), obligately aerobic bacteria (e.g., Pseudomonas species), samples with small particles or intense colour that hamper pour-plate reading, and when colony-type differentiation is required. The amendment notes limited suitability for some fermented foods and certain animal feeds; alternative media or incubation conditions may be more appropriate for those products.

Applications and who uses it

• Quality control and microbiology laboratories performing routine total viable counts for foods and feed.
• Food safety and QA/QC teams in food manufacturing (fresh, chilled, frozen, dried foods).
• Feed producers and animal-health labs monitoring feed hygiene.
• Environmental monitoring programs in food and feed production and handling facilities.
• Primary producers and regulators requiring standardized colony-count methods for compliance and surveillance.

Related standards and context

• This amendment is part of the ISO 4833 series (Microbiology of the food chain). It was approved by CEN and is to be implemented as a national standard across CEN member states. The document emphasizes minimizing deviations from the horizontal method so results remain comparable across applications.

Keywords: EN ISO 4833-2:2013/A1:2022, colony count 30 °C, surface plating technique, horizontal method, enumeration of microorganisms, food microbiology, spiral plater, cfu/ml, cfu/g.

Standards Content (Sample)

SLOVENSKI STANDARD
01-april-2022
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje števila
mikroorganizmov - 2. del: Štetje kolonij pri 30 °C s tehniko nasajanja na površino -
Dopolnilo A1: Razlaga področja uporabe (ISO 4833-2:2013/Amd 1:2022)
Microbiology of the food chain - Horizontal method for the enumeration of
microorganisms - Part 2: Colony count at 30 °C by the surface plating technique -
Amendment 1: Clarification of scope (ISO 4833-2:2013/Amd 1:2022)
Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zur Zählung von
Mikroorganismen - Teil 2: Koloniezählung bei 30 °C mittels Oberflächenverfahren -
Änderung 1: Klarstellung des Anwendungsbereichs (ISO 4833-2:2013/Amd 1:2022)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des
micro-organismes - Partie 2: Comptage des colonies à 30 °C par la technique
d'ensemencement en surface - Amendement 1: Clarification du domaine d'application
(ISO 4833-2:2013/Amd 1:2022)
Ta slovenski standard je istoveten z: EN ISO 4833-2:2013/A1:2022
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EN ISO 4833-2:2013/A1
EUROPEAN STANDARD
NORME EUROPÉENNE
January 2022
EUROPÄISCHE NORM
ICS 07.100.30
English Version
Microbiology of the food chain - Horizontal method for the
enumeration of microorganisms - Part 2: Colony count at
30 °C by the surface plating technique - Amendment 1:
Clarification of scope (ISO 4833-2:2013/Amd 1:2022)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie der Lebensmittelkette - Horizontales
horizontale pour le dénombrement des micro- Verfahren zur Zählung von Mikroorganismen - Teil 2:
organismes - Partie 2: Comptage des colonies à 30 °C Koloniezählung bei 30 °C mittels Oberflächenverfahren
par la technique d'ensemencement en surface - - Änderung 1: Klarstellung des Anwendungsbereichs
Amendement 1: Clarification du domaine d'application (ISO 4833-2:2013/Amd 1:2022)
(ISO 4833-2:2013/Amd 1:2022)
This amendment A1 modifies the European Standard EN ISO 4833-2:2013; it was approved by CEN on 1 January 2022.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for inclusion of
this amendment into the relevant national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This amendment exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the
same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 4833-2:2013/A1:2022 E
worldwide for CEN national Members.

Contents Page
European foreword . 3

European foreword
This document (EN ISO 4833-2:2013/A1:2022) has been prepared by Technical Committee ISO/TC 34
"Food products" in collaboration with Technical Committee CEN/TC 463 “Microbiology of the food
chain” the secretariat of which is held by AFNOR.
This Amendment to the European Standard EN ISO 4833-2:2013 shall be given the status of a national
standard, either by publication of an identical text or by endorsement, at the latest by July 2022, and
conflicting national standards shall be withdrawn at the latest by July 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 4833-2:2013/Amd 1:2022 has been approved by CEN as EN ISO 4833-2:2013/A1:2022
without any modification.
INTERNATIONAL ISO
STANDARD 4833-2
First edition
2013-09-01
AMENDMENT 1
2022-01
Microbiology of the food chain —
Horizontal method for the
enumeration of microorganisms —
Part 2:
Colony count at 30 °C by the surface
plating technique
AMENDMENT 1: Clarification of scope
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
le dénombrement des micro-organismes —
Partie 2: Comptage des colonies à 30 °C par la technique
d'ensemencement en surface
AMENDEMENT 1: Clarification du domaine d'application
Reference number
ISO 4833-2:2013/Amd.1:2022(E)
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