SIST EN ISO 11085:2010
(Main)Cereals, cereals-based products and animal feeding stuffs - Determination of crude fat and total fat content by the Randall extraction method (ISO 11085:2008)
Cereals, cereals-based products and animal feeding stuffs - Determination of crude fat and total fat content by the Randall extraction method (ISO 11085:2008)
This International Standard specifies procedures for the determination of the fat content of cereals, cerealbased products, and animal feeding stuffs. These procedures are not applicable to oilseeds and oleaginous fruits.
The choice of procedure to be used depends on the nature and composition of the material analysed and the reason for carrying out the analysis.
Procedure A is a method for the determination of directly extractable crude fats, applicable to all materials, except those included within the scope of procedure B.
Procedure B is a method for the determination of total fats, applicable to all materials from which the oils and fats cannot be completely extracted without prior hydrolysis.
NOTE Most cereals, as well as feeds of animal origin, yeasts, potato protein, compound feeds with milk products, glutens and products subjected to processes such as extrusion, flaking and heating, yield significantly higher total fat contents when tested by procedure B than by procedure A. See Annex B.
Getreide, Getreideerzeugnisse und Futtermittel - Bestimmung des Rohfettgehalts und des Gesamtfettgehalts mit dem Extraktionsverfahren nach Randall (ISO 11085:2008)
Diese Internationale Norm legt Verfahren zur Bestimmung des Fettgehalts von Getreide, Getreideerzeug-nissen und Futtermitteln fest. Auf Ölsaaten und ölhaltige Früchte sind diese Verfahren nicht anwendbar.
Die Auswahl des anzuwendenden Verfahrens hängt von der Art und Zusammensetzung des zu analysie-renden Materials und dem Grund für die Durchführung der Analyse ab.
Verfahren A dient zur Bestimmung des direkt extrahierbaren Rohfettgehalts und ist auf alle Materialien mit Ausnahme der im Anwendungsbereich von Verfahren B angegebenen Materialien anwendbar.
Verfahren B dient zur Bestimmung des Gesamtfettgehalts und ist auf alle Materialien anwendbar, aus denen Öle und Fette ohne vorherige Hydrolyse nicht vollständig extrahiert werden können.
ANMERKUNG Die meisten Getreide sowie Futtermittel tierischen Ursprungs, Hefen, Kartoffelprotein, Mischfuttermittel mit Milcherzeugnissen, Gluten und Erzeugnisse, die durch Pressvorgänge, Flockenbildung und unter Erwärmung ver¬arbeitet werden, ergeben bei Prüfung nach Verfahren B merklich höhere Gesamtfettgehalte als bei Prüfung nach Verfahren A. Siehe Anhang B.
Céréales, produits céréaliers et aliments des animaux - Détermination de la teneur en matières grasses brutes et en matières grasses totales par la méthode d'extraction de Randall (ISO 11085:2008)
L'ISO 11085:2008 spécifie des modes opératoires pour la détermination de la teneur en matières grasses des céréales, des produits céréaliers et des aliments pour animaux. Ces modes opératoires ne sont pas applicables aux graines et aux fruits oléagineux.
Le choix de l'un ou l'autre des modes opératoires à utiliser dépend de la nature et de la composition du produit analysé, ainsi que de l'objet de l'analyse.
Le mode opératoire A est une méthode de détermination des matières grasses brutes directement extractibles, applicable à tous les produits sauf ceux relevant du domaine d'application du mode opératoire B.
Le mode opératoire B est une méthode de détermination des matières grasses totales, applicable à tous les produits dont il n'est pas possible d'extraire complètement les huiles et graisses sans procéder à une hydrolyse préalable.
Žito in žitni proizvodi ter krma - Določevanje surove maščobe in skupne vrednosti maščob z Randallovo ekstrakcijsko metodo (ISO 11085:2008)
Ta mednarodni standard določa postopke za določevanje vsebnosti maščob v žitih, žitnih proizvodih ter krmah. Ti postopki ne veljajo za oljnice in oljne plodove. Izbira postopka je odvisna od vrste in sestave materiala, ki se ga analizira ter namena izvedbe analize. Postopek A je metoda za določevanje direktno ekstrahiranih surovih maščob, ki se uporablja pri vseh materialih razen tistih, vključenih v obsega uporabe postopka B. Postopek B je metoda za določevanje skupnih maščob, ki se uporablja za vse materiale iz katerih se olja in maščobe ne morejo popolnoma ekstrahirati brez predhodne hidrolize. OPOMBA: Večina žit, kot tudi vsa krmila živalskega porekla, kvasi, krompirjevi proteini, krmne mešanice z mlečnimi proizvodi, gluteni in proizvodi, izpostavljeni s procesi kot so iztiskanje, kosmičenje in segrevanje, ima precej višjo skupno vrednost maščob, kadar se so preskušene s postopkom B v primerjavi s postopkom A. Glej dodatek B
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 11085:2010
01-september-2010
äLWRLQåLWQLSURL]YRGLWHUNUPD'RORþHYDQMHVXURYHPDãþREHLQVNXSQHYUHGQRVWL
PDãþRE]5DQGDOORYRHNVWUDNFLMVNRPHWRGR ,62
Cereals, cereals-based products and animal feeding stuffs - Determination of crude fat
and total fat content by the Randall extraction method (ISO 11085:2008)
Getreide, Getreideerzeugnisse und Futtermittel - Bestimmung des Rohfettgehalts und
des Gesamtfettgehalts mit dem Extraktionsverfahren nach Randall (ISO 11085:2008)
Céréales, produits céréaliers et aliments des animaux - Détermination de la teneur en
matières grasses brutes et en matières grasses totales par la méthode d'extraction de
Randall (ISO 11085:2008)
Ta slovenski standard je istoveten z: EN ISO 11085:2010
ICS:
65.120 Krmila Animal feeding stuffs
67.060 äLWDVWURþQLFHLQSURL]YRGLL] Cereals, pulses and derived
QMLK products
SIST EN ISO 11085:2010 en
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
SIST EN ISO 11085:2010
---------------------- Page: 2 ----------------------
SIST EN ISO 11085:2010
EUROPEAN STANDARD
EN ISO 11085
NORME EUROPÉENNE
EUROPÄISCHE NORM
April 2010
ICS 67.060
English Version
Cereals, cereals-based products and animal feeding stuffs -
Determination of crude fat and total fat content by the Randall
extraction method (ISO 11085:2008)
Céréales, produits céréaliers et aliments des animaux - Getreide, Getreideerzeugnisse und Futtermittel -
Détermination de la teneur en matières grasses brutes et Bestimmung des Rohfettgehalts und des
en matières grasses totales par la méthode d'extraction de Gesamtfettgehalts mit dem Extraktionsverfahren nach
Randall (ISO 11085:2008) Randall (ISO 11085:2008)
This European Standard was approved by CEN on 13 March 2010.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2010 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 11085:2010: E
worldwide for CEN national Members.
---------------------- Page: 3 ----------------------
SIST EN ISO 11085:2010
EN ISO 11085:2010 (E)
Contents Page
Foreword .3
2
---------------------- Page: 4 ----------------------
SIST EN ISO 11085:2010
EN ISO 11085:2010 (E)
Foreword
The text of ISO 11085:2008 has been prepared by Technical Committee ISO/TC 34 “Food products” of the
International Organization for Standardization (ISO) and has been taken over as EN ISO 11085:2010 by
Technical Committee CEN/TC 338 “Cereal and cereal products” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by October 2010, and conflicting national standards shall be withdrawn at
the latest by October 2010.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 11085:2008 has been approved by CEN as a EN ISO 11085:2010 without any modification.
3
---------------------- Page: 5 ----------------------
SIST EN ISO 11085:2010
---------------------- Page: 6 ----------------------
SIST EN ISO 11085:2010
INTERNATIONAL ISO
STANDARD 11085
First edition
2008-05-01
Corrected version
2009-03-15
Cereals, cereals-based products and
animal feeding stuffs — Determination
of crude fat and total fat content by the
Randall extraction method
Céréales, produits céréaliers et aliments des animaux — Détermination
de la teneur en matières grasses brutes et en matières grasses totales
par la méthode d'extraction de Randall
Reference number
ISO 11085:2008(E)
©
ISO 2008
---------------------- Page: 7 ----------------------
SIST EN ISO 11085:2010
ISO 11085:2008(E)
PDF disclaimer
This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but
shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In
downloading this file, parties accept therein the responsibility of not infringing Adobe's licensing policy. The ISO Central Secretariat
accepts no liability in this area.
Adobe is a trademark of Adobe Systems Incorporated.
Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation
parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by ISO member bodies. In
the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below.
COPYRIGHT PROTECTED DOCUMENT
© ISO 2008
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or
ISO's member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail copyright@iso.org
Web www.iso.org
Published in Switzerland
ii © ISO 2008 – All rights reserved
---------------------- Page: 8 ----------------------
SIST EN ISO 11085:2010
ISO 11085:2008(E)
Contents Page
Foreword. iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions. 1
4 Principle. 2
5 Reagents. 2
6 Apparatus . 2
7 Sampling. 3
8 Procedure . 3
8.1 Preparation of the test sample . 3
8.2 Test portion . 3
8.3 Preliminary extraction . 3
8.4 Hydrolysis. 4
8.5 Extraction . 5
9 Calculation and expression of results. 5
9.1 Determination with preliminary extraction. 5
9.2 Determination without preliminary extraction . 6
10 Precision. 6
10.1 Interlaboratory test . 6
10.2 Repeatability. 6
10.3 Reproducibility. 6
10.4 Critical difference. 6
10.5 Measurement uncertainty . 7
11 Test report . 8
Annex A (informative) Results of an interlaboratory test. 9
Annex B (informative) Comparison of fat contents for the samples used in the interlaboratory test . 14
Bibliography . 16
© ISO 2008 – All rights reserved iii
---------------------- Page: 9 ----------------------
SIST EN ISO 11085:2010
ISO 11085:2008(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 11085 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 4, Cereals
and pulses, in collaboration with SC 10, Animal feeding stuffs.
ISO 11085 cancels and replaces ISO 7302:1982, which has been technically revised.
This corrected version of ISO 11085:2008 incorporates the following correction: on p. 10, Table A.1, column 8,
last row, “0,384” has been deleted and “0,891” inserted.
iv © ISO 2008 – All rights reserved
---------------------- Page: 10 ----------------------
SIST EN ISO 11085:2010
INTERNATIONAL STANDARD ISO 11085:2008(E)
Cereals, cereals-based products and animal feeding stuffs —
Determination of crude fat and total fat content by the Randall
extraction method
1 Scope
This International Standard specifies procedures for the determination of the fat content of cereals, cereal-
based products, and animal feeding stuffs. These procedures are not applicable to oilseeds and oleaginous
fruits.
The choice of procedure to be used depends on the nature and composition of the material analysed and the
reason for carrying out the analysis.
Procedure A is a method for the determination of directly extractable crude fats, applicable to all materials,
except those included within the scope of procedure B.
Procedure B is a method for the determination of total fats, applicable to all materials from which the oils and
fats cannot be completely extracted without prior hydrolysis.
NOTE Most cereals, as well as feeds of animal origin, yeasts, potato protein, compound feeds with milk products,
glutens and products subjected to processes such as extrusion, flaking and heating, yield significantly higher total fat
contents when tested by procedure B than by procedure A. See Annex B.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
ISO 3696, Water for analytical laboratory use — Specification and test methods
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
crude fat content
mass fraction of substances extracted from the sample by procedure A specified in this International Standard
NOTE The crude fat content is expressed as a percentage mass fraction.
3.2
total fat content
mass fraction of substances extracted from the sample by procedure B specified in this International Standard
NOTE The total fat content is expressed as a percentage mass fraction.
© ISO 2008 – All rights reserved 1
---------------------- Page: 11 ----------------------
SIST EN ISO 11085:2010
ISO 11085:2008(E)
4 Principle
Fat is extracted using light petroleum as a solvent and the Randall modification of the Soxhlet method. The
test portion is submerged in boiling solvent prior to rinsing in cold solvent, reducing the time needed for
extraction. The solvent dissolves fats, oils, pigments and other soluble substances. After extraction, the
solvent is evaporated and recovered by condensation. The resulting fat residue is determined gravimetrically
after drying.
For total fat determination, the sample is treated under heating with hydrochloric acid. Hydrolysis makes
chemically or mechanically bound fats accessible to solvent extraction. The mixture is cooled and filtered. The
residue is washed and dried and submitted to the above extraction procedure.
For total fat determinations of samples with a “high” fat content (i.e. at least 100 g/kg), a preliminary extraction
is performed before applying procedure B.
5 Reagents
Use only reagents of recognized analytical grade.
5.1 Water, complying with the requirements of at least grade 3 of ISO 3696.
5.2 Light petroleum (petroleum ether), consisting mainly of hydrocarbons with six carbon atoms, boiling
range 30 °C to 60 °C. The bromine value shall be less than 1. The evaporation residue shall be less than
20 mg/l.
5.3 Glass beads, of diameter 5 mm to 6 mm or silicon carbide chips.
5.4 Hydrochloric acid, c(HCl) = 3 mol/l.
1)
5.5 Filtration aid, e.g. diatomaceous earth , boiled for 30 min in hydrochloric acid, c(HCl) = 6 mol/l, washed
with water (5.1) until acid-free, then dried at 130 °C.
5.6 Acetone.
5.7 Cotton wool, defatted.
6 Apparatus
Usual laboratory apparatus and, in particular, the following.
6.1 Solvent extraction system, consisting of a 2-stage Randall extraction process unit enabling solvent
2)
recovery, fitted with fluoroelastomer or polytetrafluoroethylene seals compatible with petroleum ether.
6.2 Hydrolysis apparatus I, multiple position unit enabling boiling with acid, compatible with the solvent
extraction system (6.1), used for hydrolysis according to 8.4.1.
6.3 Hydrolysis apparatus II, consisting of either a beaker of capacity 400 ml and, as a cover, a watch
glass of appropriate diameter, or a conical flask of capacity 300 ml with a reflux condenser, used for hydrolysis
according to 8.4.2.
1) Celite® is an example of a suitable product available commercially. This information is given for the convenience of
users of this International Standard, and does not constitute an endorsement by ISO of this product.
2) Viton® is an example of a suitable product available commercially. This information is given for the convenience of
users of this International Standard, and does not constitute an endorsement by ISO of this product.
2 © ISO 2008 – All rights reserved
---------------------- Page: 12 ----------------------
SIST EN ISO 11085:2010
ISO 11085:2008(E)
6.4 Drying oven, capable of being maintained at (103 ± 2) °C.
6.5 Microwave oven, with defrost setting.
6.6 Desiccator, containing an efficient desiccant.
6.7 Extraction thimbles, of cellulose, free from petroleum ether-extractable products, and stand to hold
thimbles.
6.8 Extraction cups, of aluminium or glass, compatible with the solvent extraction system (6.1).
6.9 Glass thimbles for hydrolysis.
6.10 Analytical balance, readable to the nearest 0,1 mg.
6.11 Mill or grinder, fitted with a 1 mm screen or for samples with a fat mass fraction between 15 % and
20 %, a water-cooled knife mill.
7 Sampling
A representative sample should have been sent to the laboratory. It should not have been damaged or
changed during transport and storage.
Sampling is not part of the method specified in this International Standard. Recommended sampl
...
Questions, Comments and Discussion
Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.