EN ISO 6785:2007
(Main)Milk and milk products - Detection of Salmonella spp. (ISO 6785:2001)
Milk and milk products - Detection of Salmonella spp. (ISO 6785:2001)
ISO 6785|IDF 93:2001 specifies a method for the detection of Salmonella spp. in milk and milk products.
Milch und Milcherzeugnisse - Nachweis von Salmonella spp. (ISO 6785:2001)
Lait et produits laitiers - Recherche de Salmonella spp. (ISO 6785:2001)
L'ISO 6785|FIL 93:2001 spécifie une méthode pour la recherche de Salmonella spp. dans le lait et les produits laitiers.
Mleko in mlečni proizvodi - Ugotavljanje prisotnosti vrst Salmonella (ISO 6785:2001)
General Information
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Standards Content (Sample)
SLOVENSKI STANDARD
01-oktober-2007
0OHNRLQPOHþQLSURL]YRGL8JRWDYOMDQMHSULVRWQRVWLYUVW6DOPRQHOOD,62
Milk and milk products - Detection of Salmonella spp. (ISO 6785:2001)
Milch und Milcherzeugnisse - Nachweis von Salmonella spp. (ISO 6785:2001)
Lait et produits laitiers - Recherche de Salmonella spp. (ISO 6785:2001)
Ta slovenski standard je istoveten z: EN ISO 6785:2007
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.100.01 0OHNRLQPOHþQLSURL]YRGLQD Milk and milk products in
VSORãQR general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 6785
NORME EUROPÉENNE
EUROPÄISCHE NORM
June 2007
ICS 07.100.30
English Version
Milk and milk products - Detection of Salmonella spp. (ISO
6785:2001)
Lait et produits laitiers - Recherche de Salmonella spp. Milch und Milcherzeugnisse - Nachweis von Salmonella
(ISO 6785:2001) spp. (ISO 6785:2001)
This European Standard was approved by CEN on 19 May 2007.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36 B-1050 Brussels
© 2007 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6785:2007: E
worldwide for CEN national Members.
Foreword
The text of ISO 6785:2001 has been prepared by Technical Committee ISO/TC 34 "Agricultural
food products” of the International Organization for Standardization (ISO) and has been taken
over as EN ISO 6785:2007 by Technical Committee CEN/TC 302 "Milk and milk products -
Methods of sampling and analysis", the secretariat of which is held by NEN.
This European Standard shall be given the status of a national standard, either by publication of
an identical text or by endorsement, at the latest by December 2007, and conflicting national
standards shall be withdrawn at the latest by December 2007.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of
the following countries are bound to implement this European Standard: Austria, Belgium,
Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United
Kingdom.
Endorsement notice
The text of ISO 6785:2001 has been approved by CEN as EN ISO 6785:2007 without any
modifications.
INTERNATIONAL ISO
STANDARD 6785
IDF
Second edition
2001-05-15
Milk and milk products — Detection of
Salmonella spp.
Lait et produits laitiers — Recherche de Salmonella spp.
Reference numbers
ISO 6785:2001(E)
IDF 93:2001(E)
©
ISO and IDF 2001
ISO 6785:2001(E)
IDF 93:2001(E)
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ii © ISO and IDF 2001 – All rights reserved
ISO 6785:2001(E)
IDF 93:2001(E)
Contents Page
1 Scope . 1
2 Normative reference . 1
3 Terms and definitions . 1
4 Principle . 1
4.1 General . 1
4.2 Pre-enrichment in non-selective liquid medium . 1
4.3 Enrichment in selective liquid media . 1
4.4 Streaking out and recognition . 2
4.5 Confirmation . 2
5 Culture media, reagents and sera . 2
6 Apparatus and glassware . 12
7 Sampling . 13
8 Preparation of test sample . 13
9 Procedure . 13
9.1 Safety precautions . 13
9.2 Test portion and pre-enrichment . 13
9.3 Enrichment . 14
9.4 Streaking out and recognition . 14
9.5 Confirmation . 15
10 Control cultures . 18
11 Expression of results . 18
12 Safety precautions . 18
13 Test report . 19
Annexes
A Diagram of procedure . 20
B Specification for brilliant green. 21
C Standard method for streaking agar plates. 22
Bibliography. 23
iii
ISO 6785:2001(E)
IDF 93:2001(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical
committees. Each member body interested in a subject for which a technical committee has been established has
the right to be represented on that committee. International organizations, governmental and non-governmental, in
liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical
Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 3.
Draft International Standards adopted by the technical committees are circulated to the member bodies for voting.
Publication as an International Standard requires approval by at least 75 % of member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this International Standard may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights.
International Standard ISO 6785�IDF 93 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with
AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International.
This second edition cancels and replaces the first edition (ISO 6785:1985), which has been technically revised.
Annexes A and B form a normative part of this International Standard. Annex C is for information only.
iv
ISO 6785:2001(E)
IDF 93:2001(E)
Foreword
IDF (the International Dairy Federation) is a worldwide federation of the dairy sector with a National Committee in
every member country. Every National Committee has the right to be represented on the IDF Standing Committees
carrying out the technical work. IDF collaborates with ISO and AOAC International in the development of standard
methods of analysis and sampling for milk and milk products.
Draft International Standards adopted by the Action Teams and Standing Committees are circulated to the National
Committees for voting. Publication as an International Standard requires approval by at least 50 % of National
Committees casting a vote.
International Standard ISO 6785�IDF 93 was prepared by Technical Committee ISO/TC 34, Food products,
Subcommittee SC 5, Milk and milk products, and the International Dairy Federation (IDF), in collaboration with
AOAC International. It is being published jointly by ISO and IDF and separately by AOAC International.
All work was carried out by the Joint ISO/IDF/AOAC Action Team on Harmonization, of the Standing Committee on
Microbial methods of analysis, under the aegis of its project leader, Mr. H. Becker (DE).
This fourth edition cancels and replaces the third edition (IDF 93:1995).
v
ISO 6785:2001(E)
INTERNATIONAL STANDARD
IDF 93:2001(E)
Milk and milk products — Detection of Salmonella spp.
1 Scope
This International Standard specifies a method for the detection of Salmonella spp. in milk and milk products.
2 Normative reference
The following normative document contains provisions which, through reference in this text, constitute provisions of
this International Standard. For dated references, subsequent amendments to, or revisions of, any of these
publications do not apply. However, parties to agreements based on this International Standard are encouraged to
investigate the possibility of applying the most recent edition of the normative document indicated below. For undated
references, the latest edition of the normative document referred to applies. Members of ISO and IEC maintain
registers of currently valid International Standards.
ISO 8261�IDF 122, Milk and milk products — General guidance for the preparation of test samples, initial suspensions
and decimal dilutions for microbiological examination.
3 Terms and definitions
For the purposes of this International Standard, the following terms and definitions apply.
3.1
Salmonella
microorganisms which form typical colonies on solid selective media and which display the biochemical and
serological characteristics described when tests are carried out in accordance with this International Standard
3.2
detection of Salmonella
detection of the presence or absence of these microorganisms, in a particular mass or volume, when tests are
carried o
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