EN ISO 4833-1:2013

Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique (ISO 4833-1:2013)

ISO 4833-1:2013 specifies a horizontal method for enumeration of microorganisms that are able to grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to: a) products intended for human consumption and for animal feed; b) environmental samples in the area of food and feed production and handling.
ISO 4833-1:2013 is applicable to: 1) products that require a reliable count when a low limit of detection is specified (below 102/g or 102/ml for liquid samples or below 103/g for solid samples); 2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk and milk products likely to contain spreading Bacillus spp.
The applicability of ISO 4833-1:2013 to the examination of certain fermented food and animal feeds is limited and other media or incubation conditions can be more appropriate. However, this method can be applied to such products even though it is possible that the predominant microorganisms in those products are not detected effectively.
For some matrices, the method specified in ISO 4833-1:2013 can give different results to those obtained using the method specified in ISO 4833‑2.

Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zur Zählung von Mikroorganismen - Teil 1: Koloniezählung bei 30 °C mittels Gussplattenverfahren (ISO 4833-1:2013)

Dieser Teil von ISO 4833 legt ein horizontales Verfahren für die Zählung von nach aerober Bebrütung bei 30 °C in einem festen Medium wachsenden und Kolonien bildenden Mikroorganismen fest. Das Verfahren gilt für:
a) Produkte, die für den menschlichen Verzehr oder als Futtermittel bestimmt sind;
b) Umgebungsproben im Bereich der Herstellung und Handhabung von Lebensmitteln und Futtermitteln.
Der vorliegende Teil von ISO 4833 gilt für:
1) Produkte, bei denen eine zuverlässige Keimzählung erforderlich ist, wenn eine niedrige Nachweisgrenze (unterhalb 102/g bzw. 102/ml für flüssige Proben und unter 103/g für feste Proben) gilt;
2) Produkte, bei denen zu erwarten ist, dass sie schwärmende Kolonien (Laufkolonien) enthalten, die Kolonien anderer Organismen verschleiern, z. B. Milch und Milchprodukte, die wahrscheinlich schwärmende Bacillus spp. enthalten.
Die Anwendbarkeit dieses Teils von ISO 4833 auf die Untersuchung bestimmter fermentierter Lebens- und Futtermittel ist begrenzt und andere Medien oder Bebrütungsbedingungen sind möglicherweise geeigneter. Dennoch kann dieses Verfahren auf derartige Produkte angewendet werden, selbst wenn die Möglichkeit besteht, dass die vorherrschenden Mikroorganismen in diesen Produkten nicht effektiv nachgewiesen werden.
Bei einigen Matrices kann das in diesem Teil von ISO 4833 festgelegte Verfahren zu anderen Ergebnissen führen, als sie unter Anwendung des in ISO 4833-2 festgelegten Verfahrens erhalten werden.

Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des micro-organismes - Partie 1: Comptage des colonies à 30 degrés C par la technique d'ensemencement en profondeur (ISO 4833-1:2013)

L'ISO 4833-1:2013 spécifie une méthode horizontale de dénombrement des micro-organismes capables de se développer et de former des colonies dans un milieu solide après incubation aérobie à 30 °C. La méthode est applicable: a) aux produits destinés à la consommation humaine et aux aliments pour animaux; b) aux échantillons d'environnement dans le domaine de la production d'aliments destinés à l'homme ou aux animaux et de la préparation des aliments.
L'ISO 4833-1:2013 est applicable: 1) aux produits qui exigent un comptage fiable lorsqu'une limite inférieure de détection est spécifiée (inférieure à 102/g ou 102/ml pour des échantillons liquides ou inférieure à 103/g pour des échantillons solides); 2) aux produits supposés contenir des colonies envahissantes qui masquent les colonies d'autres organismes, par exemple le lait et les produits laitiers susceptibles de contenir diverses espèces envahissantes de Bacillus.
L'ISO 4833-1:2013 peut ne pas être adaptée à l'analyse de certains aliments fermentés et aliments pour animaux et d'autres milieux ou conditions d'incubation peuvent être plus appropriés. Toutefois, cette méthode peut être appliquée à de tels produits même si elle ne détecte pas totalement les micro-organismes présents en majorité dans ces produits.
Pour certaines matrices, la méthode spécifiée dans l'ISO 4833-1:2013 peut donner des résultats différents de ceux obtenus avec la méthode spécifiée dans l'ISO 4833-2.

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje števila mikroorganizmov - 1. del: Štetje kolonij pri 30 °C s tehniko prelivanja (ISO 4833-1:2013)

Ta del standarda ISO 4833 določa horizontalno metodo za ugotavljanje števila mikroorganizmov, ki lahko rastejo in tvorijo kolonije na trdem mediju po aerobni inkubaciji pri 30 °C. Ta metoda se uporablja za: a) izdelke za prehrano ljudi in živalsko krmo; b) okoljske vzorce v območju proizvodnje in oskrbe s hrano in krmo. Ta del standarda ISO 4833 se uporablja za: 1) izdelke, ki potrebujejo zanesljivo štetje, ko je določena nizka mejna zaznavna vrednost (pod 102/g ali 102/ml za tekoče vzorce ali pod 103/g za trde vzorce); 2) izdelke, za katere se pričakuje, da vsebujejo kolonije, ki se razraščajo in zakrivajo kolonije drugih organizmov, npr. mleko in mlečni izdelki bodo verjetno vsebovali Bacillus spp., ki se razrašča. Uporaba tega dela standarda ISO 4833 za pregledovanje nekaterih vrst fermentirane hrane in živalske krme je omejena, drugi mediji ali inkubacijski pogoji pa so morda bolj primerni. Toda ta metoda se lahko uporabi za te izdelke, čeprav se glavnih mikroorganizmov v teh izdelkih morda ne da učinkovito zaznati. Pri nekaterih matricah lahko metoda, opisana v temu delu standarda ISO 4833, da drugačne rezultate od rezultatov, pridobljenih z metodo, opisano v standardu ISO 4833-2.

General Information

• Status: Published
• Publication Date: 03-Sep-2013
• ICS: 07.100.30 - Food microbiology
• ICS: 65.120 - Animal feeding stuffs
• Technical Committee: CEN/TC 463 - Microbiology of the food chain
• Drafting Committee: CEN/TC 463 - Microbiology of the food chain
• Current Stage: 6060 - Definitive text made available (DAV) - Publishing
• Start Date: 04-Sep-2013
• Completion Date: 04-Sep-2013
• Ref Project: SIST EN ISO 4833-1:2013 - Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique (ISO 4833-1:2013)

Relations

• Replaces: EN ISO 4833:2003 - Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of microorganisms - Colony-count technique at 30 degrees C (ISO 4833:2003)
• Effective Date: 08-Jun-2022
• Amended By: EN ISO 4833-1:2013/A1:2022 - Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 °C by the pour plate technique - Amendment 1: Clarification of scope (ISO 4833-1:2013/Amd 1:2022)
• Effective Date: 16-Aug-2017
• Corrected By: EN ISO 4833-2:2013/AC:2014 - Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating technique - Technical Corrigendum 1 (ISO 4833-2:2013/Cor 1:2014)
• Effective Date: 22-Jan-2014

Standards Content (Sample)

SLOVENSKI STANDARD
01-december-2013
1DGRPHãþD
SIST EN ISO 4833:2003
Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje števila
mikroorganizmov - 1. del: Štetje kolonij pri 30 °C s tehniko prelivanja (ISO 4833-
1:2013)
Microbiology of the food chain - Horizontal method for the enumeration of
microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique (ISO
4833-1:2013)
Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zur Zählung
von Mikroorganismen - Teil 1: Koloniezählverfahren bei 30 °C mittels
Gussplattenverfahren (ISO 4833-1:2013)
Microbiologie de la chaîne alimentaire - Méthode horizontale pour le dénombrement des
micro-organismes - Partie 1: Comptage des colonies à 30 degrés C par la technique
d'ensemencement en profondeur (ISO 4833-1:2013)
Ta slovenski standard je istoveten z: EN ISO 4833-1:2013
ICS:
07.100.30 Mikrobiologija živil Food microbiology
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 4833-1
NORME EUROPÉENNE
EUROPÄISCHE NORM
September 2013
ICS 07.100.30 Supersedes EN ISO 4833:2003
English Version
Microbiology of the food chain - Horizontal method for the
enumeration of microorganisms - Part 1: Colony count at 30
degrees C by the pour plate technique (ISO 4833-1:2013)
Microbiologie de la chaîne alimentaire - Méthode Mikrobiologie von Lebensmitteln und Futtermitteln -
horizontale pour le dénombrement des micro-organismes - Horizontales Verfahren zur Zählung von Mikroorganismen -
Partie 1: Comptage des colonies à 30 degrés C par la Teil 1: Koloniezählverfahren bei 30 °C mittels
technique d'ensemencement en profondeur (ISO 4833- Gussplattenverfahren (ISO 4833-1:2013)
1:2013)
This European Standard was approved by CEN on 26 July 2013.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2013 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 4833-1:2013: E
worldwide for CEN national Members.

Contents Page
Foreword .3

Foreword
This document (EN ISO 4833-1:2013) has been prepared by Technical Committee ISO/TC 34 "Food
products" in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the
secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by March 2014, and conflicting national standards shall be withdrawn at
the latest by March 2014.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 4833:2003, together with EN ISO 4833-2:2013.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 4833-1:2013 has been approved by CEN as EN ISO 4833-1:2013 without any modification.
INTERNATIONAL ISO
STANDARD 4833-1
First edition
2013-09-01
Microbiology of the food chain —
Horizontal method for the
enumeration of microorganisms —
Part 1:
Colony count at 30 °C by the pour plate
technique
Microbiologie de la chaîne alimentaire — Méthode horizontale pour
le dénombrement des micro-organismes —
Partie 1: Comptage des colonies à 30 °C par la technique
d’ensemencement en profondeur
Reference number
ISO 4833-1:2013(E)
©
ISO 2013
ISO 4833-1:2013(E)
© ISO 2013
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form
or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior
written permission. Permission can be requested from either ISO at the address below or ISO’s member body in the country of
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Published in Switzerland
ii © ISO 2013 – All rights reserved

ISO 4833-1:2013(E)
Contents Page
Foreword .iv
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Culture media and diluents . 2
5.1 General . 2
5.2 Diluents . 2
5.3 Agar medium: plate count agar (PCA) . 2
5.4 Overlay medium (if necessary; see 9.2.7) . 3
6 Apparatus . 4
7 Sampling . 4
8 Preparation of test sample . 4
9 Procedure. 4
9.1 Test portion, initial suspension and dilutions . 4
9.2 Inoculation and incubation . 4
9.3 Counting of colonies . 5
10 Expression of results . 5
10.1 Method of calculation . 5
10.2 Precision . 5
10.3 Interpretation of test results . 6
11 Test report . 7
Annex A (informative) Use of the critical difference for the interpretation of results .8
Bibliography . 9
ISO 4833-1:2013(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2, www.iso.org/directives.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received, www.iso.org/patents.
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
The committee responsible for this document is ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
This first edition, together with ISO 4833-2, cancels and replaces ISO 4833:2003.
ISO 4833 consists of the following parts, under the general title Microbiology of the food chain —
Horizontal method for the enumeration of microorganisms:
— Part 1: Colony count at 30 °C by the pour plate technique
— Part 2: Colony count at 30 °C by the surface plating technique
iv © ISO 2013 – All rights reserved

INTERNATIONAL STANDARD ISO 4833-1:2013(E)
Microbiology of the food chain — Horizontal method for
the enumeration of microorganisms —
Part 1:
Colony count at 30 °C by the pour plate technique
1 Scope
This part of ISO 4833 specifies a horizontal method for enumeration of microorganisms that are able to
grow and form colonies in a solid medium after aerobic incubation at 30 °C. The method is applicable to:
a) products intended for human consumption and for animal feed;
b) environmental samples in the area of food and feed production and handling.
This part of ISO 4833 is applicable to:
/g
1) products that require a reliable count when a low limit of detection is specified (below 10
2 3
or 10 /ml for liquid samples or below 10 /g for solid samples);
2) products expected to contain spreading colonies that obscure colonies of other organisms, e.g. milk
and milk products likely to contain spreading Bacillus spp.
The applicability of this
...