Nanotechnologies - Endotoxin test on nanomaterial samples for in vitro systems - Limulus amebocyte lysate (LAL) test (ISO 29701:2010)

ISO 29701:2010 describes the application of a test using Limulus amebocyte lysate (LAL) reagent for the evaluation of nanomaterials intended for cell-based in vitro biological test systems. The test is suitable for use with nanomaterial samples dispersed in aqueous media, e.g. water, serum or reaction medium, and to such media incubated with nanomaterials for an appropriate duration at 37 °C.
ISO 29701:2010 is restricted to test samples for in vitro systems, but the methods can also be adapted to nanomaterials to be administered to animals by parenteral routes.

Nanotechnologien - Endotoxinprüfung an Proben aus Nanomaterial für In-vitro-Systeme - Limulus-Amoebozyten-Lysat-Prüfung (LAL-Prüfung) (ISO 29701:2010)

Die vorliegende Internationale Norm beschreibt die Anwendung einer Prüfung mit einem Limulus-Amoebozyten-Lysat-Reagenz (LAL) für die Evaluierung von Nanomaterialien, die für biologische In vitro-Prüfsysteme auf Zellbasis bestimmt sind. Die Prüfung ist für die Anwendung mit in wässrigen Medien, z. B. Wasser, Serum oder Reaktionsmedium, dispergierten Nanomaterialproben geeignet und für derartige Medien, die für eine angemessene Dauer bei 37 °C mit Nanomaterialien inkubiert wurden.
Diese Internationale Norm ist auf Prüfobjekte für In vitro-Systeme beschränkt, jedoch können die Verfahren auch an Nanomaterialien angepasst werden, die Tieren auf parenteralen Wegen zugeführt werden.

Nanotechnologies - Essai de détection d'endotoxines sur des échantillons de nanomatériaux pour des systèmes in vitro - Essai au lysat d'amébocyte de Limule (LAL) (ISO 29701:2010)

L'ISO 29701:2010 décrit l'application d'un essai utilisant le réactif LAL (lysat d'amébocytes de limule) en vue d'évaluer des nanomatériaux destinés à des systèmes d'essai biologique sur cultures cellulaires in vitro. L'essai est approprié à une utilisation avec des échantillons de nanomatériaux dispersés dans un milieu aqueux, par exemple de l'eau, du sérum physiologique ou un milieu de réaction, ainsi qu'à des milieux incubés avec des nanomatériaux pendant un temps donné à 37 °C.
L'ISO 29701:2010 est conçue pour des échantillons destinés à des systèmes in vitro, toutefois, les méthodes peuvent également être adaptées à des nanomatériaux étant administrés à des animaux par voies parentérales.

Nanotehnologija - Endotoksinski preskus vzorcev iz nanomaterialov za sisteme in vitro - Preskus Limulus amebocyte lysate (LAL) (ISO 29701:2010)

Ta mednarodni standard določa preskus za zaznavanje in kvantifikacijo endotoksinov, ki lahko kontaminirajo vzorce v vodnih nanomaterialih za biološke preskusne sisteme in vitro z uporabo reagenta Limulus Amebocyte Lysate (LAL).

General Information

Status
Published
Publication Date
14-Sep-2010
Withdrawal Date
30-Mar-2011
Technical Committee
Current Stage
6060 - Definitive text made available (DAV) - Publishing
Start Date
15-Sep-2010
Completion Date
15-Sep-2010

Buy Standard

Standard
EN ISO 29701:2011
English language
28 pages
sale 10% off
Preview
sale 10% off
Preview
e-Library read for
1 day

Standards Content (Sample)

SLOVENSKI STANDARD
SIST EN ISO 29701:2011
01-maj-2011
Nanotehnologija - Endotoksinski preskus vzorcev iz nanomaterialov za sisteme in
vitro - Preskus Limulus amebocyte lysate (LAL) (ISO 29701:2010)
Nanotechnologies - Endotoxin test on nanomaterial samples for in vitro systems -
Limulus amebocyte lysate (LAL) test (ISO 29701:2010)
Nanotechnologien - Endotoxinprüfung an Proben aus nanomaterial für In-vitro-Systeme -
Limulus-Amoebozyten-Lysat-Prüfung (LAL-Prüfung) (ISO 29701:2010)
Nanotechnologies - Essai de détection d'endotoxines sur des échantillons de
nanomatériaux pour des systèmes in vitro - Essai au lysat d'amébocyte de Limule (LAL)
(ISO 29701:2010)
Ta slovenski standard je istoveten z: EN ISO 29701:2010
ICS:
07.120 Nanotehnologije Nanotechnologies
11.100.10 'LDJQRVWLþQLSUHVNXVQL In vitro diagnostic test
VLVWHPLLQYLWUR systems
SIST EN ISO 29701:2011 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

---------------------- Page: 1 ----------------------

SIST EN ISO 29701:2011

---------------------- Page: 2 ----------------------

SIST EN ISO 29701:2011


EUROPEAN STANDARD
EN ISO 29701

NORME EUROPÉENNE

EUROPÄISCHE NORM
September 2010
ICS 11.100.10; 07.030
English Version
Nanotechnologies - Endotoxin test on nanomaterial samples for
in vitro systems - Limulus amebocyte lysate (LAL) test (ISO
29701:2010)
Nanotechnologies - Essai de détection d'endotoxines sur Nanotechnologien - Endotoxinprüfung an Proben aus
des échantillons de nanomatériaux pour des systèmes in nanomaterial für In-vitro-Systeme - Limulus-Amoebozyten-
vitro - Essai au lysat d'amébocyte de Limule (LAL) (ISO Lysat-Prüfung (LAL-Prüfung) (ISO 29701:2010)
29701:2010)
This European Standard was approved by CEN on 22 August 2010.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,
Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.





EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2010 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 29701:2010: E
worldwide for CEN national Members.

---------------------- Page: 3 ----------------------

SIST EN ISO 29701:2011
EN ISO 29701:2010 (E)
Contents Page
Foreword .3

2

---------------------- Page: 4 ----------------------

SIST EN ISO 29701:2011
EN ISO 29701:2010 (E)
Foreword
The text of ISO 29701:2010 has been prepared by Technical Committee ISO/TC 229 “Nanotechnologies” of
the International Organization for Standardization (ISO) and has been taken over as EN ISO 29701:2010 by
Technical Committee CEN/TC 352 “Nanotechnologies” the secretariat of which is held by BSI.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by March 2011, and conflicting national standards shall be withdrawn at
the latest by March 2011.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 29701:2010 has been approved by CEN as a EN ISO 29701:2010 without any modification.

3

---------------------- Page: 5 ----------------------
...

Questions, Comments and Discussion

Ask us and Technical Secretary will try to provide an answer. You can facilitate discussion about the standard in here.