EN ISO 6341:2012
(Main)Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test (ISO 6341:2012)
Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity test (ISO 6341:2012)
ISO 6341.2012 specifies a method for the determination of the acute toxicity to Daphnia magna Straus (Cladocera, Crustacea).
This method is applicable to: chemical substances which are soluble under the conditions of the test, or can be maintained as a stable suspension or dispersion under the conditions of the test; industrial or sewage effluents; treated or untreated waste water; aqueous extracts and leachates; fresh water (surface and ground water); eluates of fresh water sediment; pore water of fresh water sediment.
Wasserbeschaffenheit - Bestimmung der Hemmung der Beweglichkeit von Daphnia magna Straus (Cladocera, Crustacea) - Akuter Toxizitäts-Test (ISO 6341:2012)
Diese Internationale Norm legt ein Verfahren zur Bestimmung der akuten Toxizität gegenüber Daphnia magna Straus (Cladocera, Crustacea) fest.
Das Verfahren ist anwendbar auf:
- Chemikalien, die unter den Testbedingungen löslich sind, oder als stabile Suspension oder Dispersion unter den Testbedingungen erhalten bleiben können;
- industrielle oder kommunale Abwässer;
- behandelte oder unbehandelte Abwässer;
- wässrige Extrakte und Sickerwasser;
- Süßwasser (Oberflächen und Grundwasser);
- Eluate von Süßwassersedimenten;
- Porenwasser von Süßwassersedimenten.
Qualité de l'eau - Détermination de l'inhibition de la mobilité de Daphnia magna Straus (Cladocera, Crustacea) - Essai de toxicité aiguë (ISO 6341:2012)
L'ISO 6341:2012 spécifie une méthode de détermination de la toxicité aiguë vis-à-vis de Daphnia magna Straus (Cladocera, Crustacea).
Cette méthode s'applique aux: a) substances chimiques solubles dans les conditions de l'essai ou pouvant être maintenues en suspension ou en dispersion stable dans les conditions de l'essai; b) effluents industriels ou domestiques; c) eaux usées traitées ou non; d) extraits aqueux et lixiviats; e) eaux douces (eaux de surface et eaux souterraines); f) éluats de sédiments d'eaux douces; g) eaux interstitielles de sédiments d'eau douce.
Kakovost vode - Določanje zaviranja gibanja Daphnia magna Straus (Cladocera, Crustacea) - Akutni preskus strupenosti (ISO 6341:2012)
Ta mednarodni standard opisuje metodo za določevanje akutne strupenosti za Daphnia magna Straus (Cladocera, Crustacea). Ta metoda se uporablja za: – kemične snovi, ki so topne pod pogoji preskusa ali se lahko pod pogoji preskusa ohranijo v stabilni suspenziji ali disperziji, – industrijske ali komunalne odplake, – obdelano ali neobdelano odpadno vodo, – vodne ekstrakte in izcedne vode, – sladko vodo (površinsko in podtalno), – izlužke sedimenta sladke vode, – porne vode svežega vodnega sedimenta.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
01-januar-2013
1DGRPHãþD
SIST EN ISO 6341:1996
SIST EN ISO 6341:1996/AC:2004
.DNRYRVWYRGH'RORþDQMH]DYLUDQMDJLEDQMD'DSKQLDPDJQD6WUDXV &ODGRFHUD
&UXVWDFHD $NXWQLSUHVNXVVWUXSHQRVWL ,62
Water quality - Determination of the inhibition of the mobility of Daphnia magna Straus
(Cladocera, Crustacea) - Acute toxicity test (ISO 6341:2012)
Wasserbeschaffenheit - Bestimmung der Hemmung der Beweglichkeit von Daphnia
magna Straus (Cladocera, Crustacea) - Akuter Toxizitäts-Test (ISO 6341:2012)
Qualité de l'eau - Détermination de l'inhibition de la mobilité de Daphnia magna Straus
(Cladocera, Crustacea) - Essai de toxicité aiguë (ISO 6341:2012)
Ta slovenski standard je istoveten z: EN ISO 6341:2012
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
EUROPEAN STANDARD
EN ISO 6341
NORME EUROPÉENNE
EUROPÄISCHE NORM
October 2012
ICS 13.060.70 Supersedes EN ISO 6341:1996
English Version
Water quality - Determination of the inhibition of the mobility of
Daphnia magna Straus (Cladocera, Crustacea) - Acute toxicity
test (ISO 6341:2012)
Qualité de l'eau - Détermination de l'inhibition de la mobilité Wasserbeschaffenheit - Bestimmung der Hemmung der
de Daphnia magna Straus (Cladocera, Crustacea) - Essai Beweglichkeit von Daphnia magna Straus (Cladocera,
de toxicité aiguë (ISO 6341:2012) Crustacea) - Akuter Toxizitäts-Test (ISO 6341:2012)
This European Standard was approved by CEN on 14 October 2012.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2012 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 6341:2012: E
worldwide for CEN national Members.
Contents Page
Foreword .3
Foreword
This document (EN ISO 6341:2012) has been prepared by Technical Committee ISO/TC 147 “Water quality”
in collaboration with Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by
DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by April 2013, and conflicting national standards shall be withdrawn at the
latest by April 2013.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 6341:1996.
According to the CEN/CENELEC Internal Regulations, the national standards organisations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom.
Endorsement notice
The text of ISO 6341:2012 has been approved by CEN as a EN ISO 6341:2012 without any modification.
INTERNATIONAL ISO
STANDARD 6341
Fourth edition
2012-10-15
Water quality — Determination of the
inhibition of the mobility of Daphnia
magna Straus (Cladocera, Crustacea) —
Acute toxicity test
Qualité de l’eau — Détermination de l’inhibition de la mobilité de Daphnia
magna Straus (Cladocera, Crustacea) — Essai de toxicité aiguë
Reference number
ISO 6341:2012(E)
©
ISO 2012
ISO 6341:2012(E)
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
ISO copyright office
Case postale 56 • CH-1211 Geneva 20
Tel. + 41 22 749 01 11
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Published in Switzerland
ii © ISO 2012 – All rights reserved
ISO 6341:2012(E)
Contents Page
Foreword .iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Test environment . 2
6 Reagents, test organisms and media . 3
7 Apparatus and materials . 4
8 Treatment and preparation of samples . 5
8.1 Special precautions for sampling, transportation, storage and treatment of water, effluent, or
aqueous extract samples to be tested . 5
8.2 Preparation of solutions of substances to be tested . 6
9 Procedure . 6
9.1 General . 6
9.2 Preliminary test . 7
9.3 Definitive test . 7
9.4 Check of the sensitivity of the Daphnia magna and conformity with the procedure . 7
9.5 Limit test . 8
10 Interpretation and validity of the results . 8
10.1 Estimation of the EC . 8
10.2 Validity criteria . 8
11 Expression of results . 8
12 Test report . 8
Annex A (informative) Preparation of the Elendt M4 medium.10
Annex B (informative) Example of graphical determination of the inhibition of mobility of Daphnia
magna by an effluent or stock solution of a substance at a concentration of 1 000 mg/l .13
Annex C (informative) General recommendations for stock culturing .16
Annex D (informative) Culturing of Daphnia magna for production of dormant eggs .17
Annex E (informative) Precision data .19
Annex F (informative) Dilution level D — Preparation of the dilution series for the determination of
the LID .20
Bibliography .22
ISO 6341:2012(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies
(ISO member bodies). The work of preparing International Standards is normally carried out through ISO
technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International
Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.
The main task of technical committees is to prepare International Standards. Draft International Standards
adopted by the technical committees are circulated to the member bodies for voting. Publication as an
International Standard requires approval by at least 75 % of the member bodies casting a vote.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights.
ISO 6341 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods.
This fourth edition cancels and replaces the third edition (ISO 6341:1996), which has been technically revised.
It also incorporates the Technical Corrigendum ISO 6341:1996/Cor. 1:1998.
iv © ISO 2012 – All rights reserved
ISO 6341:2012(E)
Introduction
This International Standard specifies a procedure for the determination of the acute toxicity of chemicals,
waters and waste waters to the water flea Daphnia magna Straus.
The evaluation of harmful effects on water quality has for several years involved the performance of biological
tests. Crustaceans are of interest from the ecotoxicological point of view because they are primary consumers
and a major component of the zooplankton in aquatic ecosystems.
The test specified in this International Standard involves the determination of the immobilization of the water
flea Daphnia magna Straus after 24 h or 48 h exposure (depending on the requirement of users or national
authorities) to the test sample under the conditions specified in this International Standard.
INTERNATIONAL STANDARD ISO 6341:2012(E)
Water quality — Determination of the inhibition of the mobility
of Daphnia magna Straus (Cladocera, Crustacea) — Acute
toxicity test
WARNING — Persons using this International Standard should be familiar with normal laboratory
practice. This document does not purport to address all of the safety problems, if any, associated with
its use. It is the responsibility of the user to establish appropriate safety and health practices and to
ensure compliance with any national regulatory conditions.
IMPORTANT — It is absolutely essential that tests conducted in accordance with this International
Standard be carried out by suitably qualified staff.
1 Scope
This International Standard specifies a method for the
...
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