EN ISO 16014-5:2012

Plastics - Determination of average molecular mass and molecular mass distribution of polymers using size-exclusion chromatography - Part 5: Method using light-scattering detection (ISO 16014-5:2012)

The standard specifies a general method for determining the average molecular mass and molecular mass distribution of polymers from using SEC-LS, i.e. size-exclusion chromatography (SEC) coupled with light scattering detection (LS). The average molecular mass and the molecular mass distribution are calculated from data of molecular mass and mass concentration determined continuously at eacht elution time. The molecular mass at each elution time is determined absolutely by combining a light scattering detector with a concentration-sensitive detector. Therefore this method is classified as an absolute method (see Clause A.1 in Annes A).

Kunststoffe - Bestimmung der durchschnittlichen Molmasse und der Molmassenverteilung von Polymeren mittels Gelpermeationschromatographie - Teil 5: Lichtstreudetektionsverfahren (ISO 16014-5:2012)

Dieser Teil von ISO 16014 legt ein allgemeines Verfahren zum Bestimmen der durchschnittlichen Molmasse und der Molmassenverteilung von Polymeren durch GPC-LS, d. h. Gelpermeationschromatographie (GPC), gekoppelt mit Messung durch Lichtstreuung (LS), fest. Die durchschnittliche Molmasse und die Molmassenverteilung werden aus den Daten für die Molmasse und die Massenkonzentrationen berechnet, die kontinuierlich mit der Elutionszeit bestimmt werden. Die Molmasse bei jeder Elutionszeit wird als Absolutwert bestimmt, indem ein Detektor für die Lichtstreuung mit einem Konzentrationsdetektor kombiniert wird. Die GPC-LS wird deshalb als ein absolutes Verfahren klassifiziert.
Zur Anwendbarkeit des Verfahrens siehe ISO 16014-1:2012, Abschnitt A.1.

Plastiques - Détermination de la masse moléculaire moyenne et de la distribution des masses moléculaires de polymères par chromatographie d'exclusion stérique - Partie 5: Méthode utilisant la détection par diffusion lumineuse (ISO 16014-5:2012)

Polimerni materiali - Določevanje povprečne molekularne mase in porazdelitev molekularne mase polimerov z uporabo izključitvene kromatografije (SEC) - 5. del: Metoda s sipanjem svetlobe (ISO 16014-5:2012)

Ta del standarda ISO 16014 določa splošno metodo za ugotavljanje povprečne molekulske mase in porazdelitev molekulske mase polimerov z uporabo izključitvene kromatografije skupaj s sipanjem svetlobe (SEC-LS). Povprečna molekulska masa in porazdelitev molekulske mase se izračunata na podlagi podatkov o molekulski masi in koncentraciji mase, ki se kontinuirano določa z elucijskim časom. Molekulska masa se za posamezni elucijski čas določi absolutno s kombinacijo sipanja svetlobe in na koncentracijo občutljivega detektorja. Zato se izključitvena kromatografija skupaj s sipanjem svetlobe šteje za absolutno metodo. Za uporabo metode glejte točko A.1 standarda ISO 16014-1:2012.

General Information

• Status: Withdrawn
• Publication Date: 30-Jun-2012
• Withdrawal Date: 11-Jun-2019
• ICS: 71.040.50 - Physicochemical methods of analysis
• ICS: 83.080.01 - Plastics in general
• Technical Committee: CEN/TC 139 - Paints and varnishes
• Drafting Committee: CEN/TC 139 - Paints and varnishes
• Current Stage: 9960 - Withdrawal effective - Withdrawal
• Completion Date: 12-Jun-2019
• Ref Project: SIST EN ISO 16014-5:2012 - Plastics - Determination of average molecular mass and molecular mass distribution of polymers using size-exclusion chromatography - Part 5: Method using light-scattering detection (ISO 16014-5:2012)

Relations

• Replaced By: EN ISO 16014-5:2019 - Plastics - Determination of average molecular weight and molecular weight distribution of polymers using size-exclusion chromatography - Part 5: Light-scattering method (ISO 16014-5:2019)
• Effective Date: 19-Jun-2019

Standards Content (Sample)

SLOVENSKI STANDARD
01-september-2012
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PROHNXODUQHPDVHSROLPHURY]XSRUDERL]NOMXþLWYHQHNURPDWRJUDILMH6(&GHO
0HWRGDVVLSDQMHPVYHWOREH,62
Plastics - Determination of average molecular mass and molecular mass distribution of
polymers using size-exclusion chromatography - Part 5: Method using light-scattering
detection (ISO 16014-5:2012)
Kunststoffe - Bestimmung der durchschnittlichen Molmasse und der
Molmassenverteilung von Polymeren mittels Gelpermeationschromatographie - Teil 5:
Lichtstreudetektionsverfahren (ISO 16014-5:2012)
Plastiques - Détermination de la masse moléculaire moyenne et de la distribution des
masses moléculaires de polymères par chromatographie d'exclusion stérique - Partie 5:
Méthode utilisant la détection par diffusion lumineuse (ISO 16014-5:2012)
Ta slovenski standard je istoveten z: EN ISO 16014-5:2012
ICS:
83.080.01 Polimerni materiali na Plastics in general
splošno
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN ISO 16014-5
NORME EUROPÉENNE
EUROPÄISCHE NORM
July 2012
ICS 83.080.01
English Version
Plastics - Determination of average molecular mass and
molecular mass distribution of polymers using size-exclusion
chromatography - Part 5: Method using light-scattering detection
(ISO 16014-5:2012)
Plastiques - Détermination de la masse moléculaire
Kunststoffe - Bestimmung der durchschnittlichen Molmasse
moyenne et de la distribution des masses moléculaires de und der Molmassenverteilung von Polymeren mittels
polymères par chromatographie d'exclusion stérique - Gelpermeationschromatographie - Teil 5:
Partie 5: Méthode utilisant la détection par diffusion Lichtstreudetektionsverfahren (ISO 16014-5:2012)
lumineuse (ISO 16014-5:2012)
This European Standard was approved by CEN on 30 June 2012.

CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.

This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same
status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania,
Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United
Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

Management Centre: Avenue Marnix 17, B-1000 Brussels
© 2012 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 16014-5:2012: E
worldwide for CEN national Members.

Contents Page
Foreword .3
Foreword
This document (EN ISO 16014-5:2012) has been prepared by Technical Committee ISO/TC 61 "Plastics" in
collaboration with Technical Committee CEN/TC 139 “Paints and varnishes” the secretariat of which is held by
DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by January 2013, and conflicting national standards shall be withdrawn at
the latest by January 2013.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech
Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece,
Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom.
Endorsement notice
The text of ISO 16014-5:2012 has been approved by CEN as a EN ISO 16014-5:2012 without any
modification.
INTERNATIONAL ISO
STANDARD 16014-5
First edition
2012-07-01
Plastics — Determination of average
molecular mass and molecular mass
distribution of polymers using size-
exclusion chromatography —
Part 5:
Method using light-scattering detection
Plastiques — Détermination de la masse moléculaire moyenne
et de la distribution des masses moléculaires de polymères par
chromatographie d’exclusion stérique —
Partie 5: Méthode utilisant la détection par diffusion lumineuse
Reference number
ISO 16014-5:2012(E)
©
ISO 2012
ISO 16014-5:2012(E)
All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means,
electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISO’s
member body in the country of the requester.
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Published in Switzerland
ii © ISO 2012 – All rights reserved

ISO 16014-5:2012(E)
Contents Page
Foreword . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Symbols . 2
5 Principle . 2
5.1 SEC . 2
5.2 Light-scattering SEC . 2
6 Reagents . 2
6.1 Eluent . 2
6.2 Reagent for column evaluation . 3
6.3 Calibration standards . 3
6.4 Reagent for flow rate marker . 3
6.5 Additives . 3
7 Apparatus . 3
7.1 General . 3
7.2 Eluent reservoir . 4
7.3 Pumping system . 4
7.4 Injector . 4
7.5 Columns . 4
7.6 Detector . 5
7.7 Tubing . 5
7.8 Temperature control . 5
7.9 Recorder and plotter . 5
7.10 Data-processing system . 5
7.11 Other components . 5
8 Procedure . 5
8.1 Preparation of calibration solutions . 5
8.2 Preparation of a solution for determining the L-point . 5
8.3 Preparation of sample solutions . 5
8.4 Preparation of solutions for column performance evaluation . 6
8.5 Setting up the apparatus . 6
8.6 Operating parameters . 6
8.7 Number of determinations . 6
9 Calibration . 6
9.1 Calibration of concentration-sensitive detector and light-scattering detector . 6
9.2 Determination of delay volume . 8
9.3 Normalization of detector sensitivity . 8
9.4 Determination of refractive index increment . 8
10 Data acquisition and processing . 8
10.1 Data acquisition . 8
10.2 Evaluation of data and correction of chromatograms . 8
10.3 Data processing . 9
11 Expression of results .10
11.1 Calibration curve .10
11.2 Calculation of average molecular mass .12
11.3 Differential molecular mass distribution curve .12
11.4 Cumulative molecular mass distribution curve .12
12 Precision .13
13 Test report .
...