SIST EN 16421:2015
(Main)Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)
Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)
This European Standard specifies procedures for determining the ability of non-metallic materials to promote the growth of microorganisms.
This standard is applicable to those materials destined to be used under various conditions for the transport and storage of water intended for human consumption.
The Standard allows for the testing of a single type of material, or a product in which only one material is in contact with water. It is unsuitable for use with assembled products where more than one material is exposed to water.
Einfluss von Materialien auf Wasser für den menschlichen Gebrauch - Förderung des mikrobiellen Wachstums
Influence des matériaux sur l'eau destinée à la consommation humaine - Stimulation de la croissance microbienne (SCM)
La présente Norme européenne spécifie des modes opératoires pour déterminer la capacité de matériaux non métalliques à favoriser la croissance de micro-organismes.
La présente norme s’applique aux matériaux destinés à être utilisés dans diverses conditions pour le transport et le stockage de l’eau destinée à la consommation humaine.
La norme prévoit de soumettre à essai des échantillons ne comportant qu’un seul type de matériau ou un produit dans lequel un seul type de matériau est en contact avec l’eau. Son utilisation ne convient pas aux produits assemblés dans lesquels plusieurs matériaux sont exposés à l’eau.
Vpliv materialov na pitno vodo - Spodbujanje mikrobiološke rasti
Ta evropski standard določa postopke za ugotavljanje zmožnosti nekovinskih materialov za spodbujanje rasti mikroorganizmov.
Ta standard se uporablja za materiale, ki so namenjeni uporabi v različnih pogojih za transport in skladiščenje pitne vode.
Standard omogoča preskušanje materiala enotnega tipa ali proizvoda, pri katerem je le en material v stiku z vodo. Ni primeren za uporabo s sestavljenimi proizvodi, pri katerih je vodi izpostavljen več kot en material.
General Information
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Vpliv materialov na pitno vodo - Spodbujanje mikrobiološke rastiEinfluss von Materialien auf Wasser für den menschlichen Gebrauch - Förderung des mikrobiellen WachstumsInfluence des matériaux sur l'eau destinée à la consommation humaine - Stimulation de la croissance microbienne (SCM)Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)67.250Materiali in predmeti v stiku z živiliMaterials and articles in contact with foodstuffs13.060.20Pitna vodaDrinking water07.100.20Mikrobiologija vodeMicrobiology of waterICS:Ta slovenski standard je istoveten z:EN 16421:2014SIST EN 16421:2015en,fr01-februar-2015SIST EN 16421:2015SLOVENSKI
STANDARD
SIST EN 16421:2015
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16421
December 2014 ICS 07.100.20 English Version
Influence of materials on water for human consumption - Enhancement of microbial growth (EMG)
Influence des matériaux sur l'eau destinée à la consommation humaine - Stimulation de la croissance microbienne (SCM)
Einfluss von Materialien auf Wasser für den menschlichen Gebrauch - Förderung des mikrobiellen Wachstums This European Standard was approved by CEN on 25 October 2014.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2014 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16421:2014 ESIST EN 16421:2015
EN 16421:2014 (E) 2 Contents Page Foreword .6 Introduction .7 1 Scope .9 2 Normative references .9 3 Terms and definitions . 10 4 Influence of materials on water for human consumption – Enhancement of microbial growth (EMG) – Method 1: Measured by Biomass Production Potential (BPP) measured by ATP . 11 4.1 General . 11 4.2 Principle . 12 4.3 Apparatus . 12 4.3.1 Requirements . 12 4.3.2 Vessels, containers, stoppers and connectors and general laboratory apparatus . 12 4.3.3 Cleaning . 12 4.3.4 Gloves . 12 4.3.5 Plates . 12 4.3.6 Test containers . 13 4.3.7 Test tubes . 13 4.3.8 Incubator (or hot room) . 13 4.3.9 Sterilisation ovens . 13 4.3.10 Membrane filters . 13 4.3.11 Autoclave . 13 4.4 Reagents . 13 4.4.1 Test water . 13 4.4.2 Dilution water . 14 4.4.3 Potassium dihydrogen orthophosphate solution . 14 4.4.4 Potassium-nitrate solution . 14 4.4.5 Sodium acetate solution . 15 4.4.6 Inoculum . 15 4.4.7 Specifications for controls . 15 4.5 Test samples and controls . 16 4.5.1 Sampling, transport and storage of test samples . 16 4.5.2 Test piece preparation . 16 4.6 Pre-treatment of test pieces . 17 4.6.1 General . 17 4.6.2 Flushing . 17 4.6.3 Stagnation . 17 4.6.4 Prewashing . 17 4.7 Test procedure . 17 4.7.1 Overview . 17 4.7.2 ATP contamination . 18 4.7.3 Preparation of the test container . 18 4.7.4 Test pieces . 18 4.7.5 Incubation . 18 4.7.6 Positive and negative controls . 18 4.7.7 Changing of test water . 19 4.7.8 Measurement of ATP concentrations . 19 4.8 Calculation of test results . 19 4.8.1 Biomass Production (BP) . 19 4.9 Reproducibility and repeatability . 22 SIST EN 16421:2015
EN 16421:2014 (E) 3 4.10 Test report . 22 4.10.1 General . 22 4.10.2 Material/Product information. 22 4.10.3 Information on the test procedure . 23 4.10.4 Test results . 23 5 Influence of materials on water for human consumption – Enhancement of microbial growth (EMG) – Method 2: Measured by biofilm volume . 23 5.1 General . 23 5.2 Principle. 24 5.3 Basic requirements . 24 5.4 Apparatus and reagents . 24 5.4.1 Disinfectant . 24 5.4.2 Test water . 24 5.4.3 Specifications for controls . 25 5.4.4 Test container and test module for pipes and hoses . 25 5.4.5 Scraper . 25 5.4.6 Centrifuge . 25 5.4.7 Glassware for centrifugation . 25 5.4.8 Flowmeter . 25 5.5 Test samples and test pieces . 25 5.5.1 Sampling, transport and storage . 25 5.5.2 Preparation of test pieces. 26 5.6 Test procedure . 27 5.6.1 General . 27 5.6.2 Test procedure in test container . 27 5.6.3 Test procedure in test module for pipes and hoses . 27 5.6.4 Preparation of the test container or test module for pipes and hoses . 28 5.6.5 Pre-treatment of test pieces . 28 5.6.6 Removal of test pieces . 28 5.6.7 Examination of test pieces . 28 5.6.8 Re-exposure after harvesting . 28 5.6.9 Centrifugation and determination of biofilm . 28 5.6.10 Examination of biofilm . 29 5.6.11 Test periods . 29 5.7 Calculation of test results. 30 5.8 Evaluation of satisfactory test performance . 31 5.9 Test report . 32 5.9.1 General . 32 5.9.2 Material/Product information. 32 5.9.3 Information on the test procedure . 33 5.9.4 Test results . 33 6 Influence of materials on water for human consumption – Enhancement of microbial growth (EMG) – Method 3: Measured by mean dissolved oxygen depletion . 34 6.1 General . 34 6.2 Principle. 34 6.3 Test premises . 34 6.3.1 General . 34 6.3.2 Safety . 34 6.4 Apparatus and reagents . 34 6.4.1 General . 34 6.4.2 Inoculum water . 34 6.4.3 Test water . 35 6.4.4 Test containers . 36 6.4.5 Dissolved oxygen meter . 37 6.4.6 30 °C Incubator/chamber . 37 6.4.7 Polyethylene bags . 37 6.4.8 Glass plates . 37 SIST EN 16421:2015
EN 16421:2014 (E) 4 6.4.9 Brass couplings . 37 6.4.10 Special glass plates . 37 6.5 Preparation of test pieces . 37 6.5.1 General . 37 6.5.2 Nature of test pieces . 37 6.6 Test piece and test container size . 37 6.6.1 General . 37 6.6.2 Test sample storage . 38 6.6.3 Special requirements . 38 6.6.4 Specifications for controls . 42 6.6.5 Test water control . 42 6.7 Test procedures . 42 6.7.1 Preparation of the test container . 42 6.7.2 Incubation . 43 6.7.3 Changing the test water . 43 6.7.4 Measurement of dissolved oxygen . 43 6.8 Calculation of results . 44 6.8.1 Calculation of the mean dissolved oxygen difference (MDOD) . 44 6.8.2 Validation of results. 44 6.8.3 Repeatability and reproducibility . 45 6.9 Test report . 45 6.9.1 General . 45 6.9.2 Material/Product information . 45 6.9.3 Information on the test procedure . 47 6.9.4 Results . 47 Annex A (normative)
Method 1: Measured by Biomass Production Potential (BPP) measured by ATP – Assessment of the suitability of test water for its use in the BPP test (biological stability and the need for addition of trace elements) . 48 Annex B (normative)
Method 1: Measured by Biomass Production Potential (BPP) measured by ATP – Protocol of ATP analysis . 50 B.1 Introduction . 50 B.2 Apparatus and glassware . 50 B.3 Reagents and auxiliary substances . 50 B.3.1 General . 50 B.3.2 Sterile water . 50 B.3.3 ATP solutions for standard dilutions and calibration curves . 50 B.3.4 Luciferin/luciferase (enzyme) solution . 51 B.3.5 ATP extraction reagent . 52 B.4 Sampling . 52 B.5 Procedure . 52 B.5.1 General . 52 B.5.2 Blank water . 52 B.5.3 Control dilutions . 52 B.5.4 Samples . 52 B.6 Expression of results . 52 B.6.1 Water samples . 52 B.6.2 Material samples . 53 B.7 Test record . 53 SIST EN 16421:2015
EN 16421:2014 (E) 5 B.8 Quality control of calibration curves . 53 Annex C (informative)
Method 1: Measured by Biomass Production Potential (BPP) measured by ATP - ATP and its measurement . 55 Annex D (informative)
Method 1: Measured by Biomass Production Potential (BPP) measured by ATP – Evaluating procedures for removal of attached biomass . 56 D.1 Introduction . 56 D.2 Procedures for removal of Attached Biomass . 56 D.2.1 General . 56 D.2.2 Swabbing . 56 D.2.3 Low energy sonication (LES) . 56 D.2.4 High energy sonication (HES) . 56 D.3 Effectiveness of removal procedure . 57 Annex E (informative) Method 1: Measured by Biomass Production Potential (BPP) measured by ATP . 58 Annex F (informative)
Method 2: Measured by biofilm volume . 59 Annex G (informative)
Method 2: Measured by biofilm volume . 60 Annex H (informative)
Method 3: Measured by mean dissolved oxygen depletion . 63 Bibliography . 65
SIST EN 16421:2015
EN 16421:2014 (E) 6 Foreword This document (EN 16421:2014) has been prepared by Technical Committee CEN/TC 164 “Water supply”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by June 2015, and conflicting national standards shall be withdrawn at the latest by June 2015. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. SIST EN 16421:2015
EN 16421:2014 (E) 7 Introduction Water intended for human consumption comes into contact with construction products during storage, transportation and distribution, including water systems inside buildings. The materials used in these products are selected on the basis of technical requirements and criteria regarding their influence on the water quality, e.g. relea
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