FprEN 17899

Water quality - Spectrophotometric determination of chlorophyll-a content by ethanol extraction for the routine monitoring of water quality

This document describes a spectrophotometric method for determining the chlorophyll-a content corrected for phaeopigments as a measure of the amount of phytoplankton for all types of surface water including marine water. The determination limit is usually 2 µg/l to 5 µg/l and is calculated by each laboratory individually. It can be as low as 0,5 µg/l using 2 l of sample (or even more) and a 50 mm cuvette.
NOTE   In some measurement programs like marine studies on time series data and ecological status/classification no correction for phaeopigments is used and acidification is omitted, e.g. as recommended by OSPAR.

Wasserbeschaffenheit - Bestimmung des Chlorophyll-a-Gehalts durch Ethanolextraktion für die routinemäßige Überwachung der Wasserqualität

Dieses Dokument legt ein spektrophotometrisches Verfahren zur Bestimmung des Chlorophyll a-Gehalts, korrigiert bezüglich Phaeopigmenten, als Maß für die Menge an Phytoplankton für alle Arten von Oberflächenwasser und Meerwasser fest. Die Bestimmungsgrenze ist gewöhnlich zwischen 2 µg/l bis 5 µg/l und wird von jedem Labor individuell berechnet. Sie kann bis zu 0,5 µg/l bei Verwendung einer Probe von 2 l (oder mehr) und einer 50 mm-Küvette betragen.
ANMERKUNG   In einigen Messprogrammen wie marinen Untersuchungen von Zeitreihendaten und des ökologischen Zustands/Klassifikation erfolgt keine Korrektur nach Phaeopigmenten und die Ansäuerung wird unterlassen, z.B. auf Empfehlung von OSPAR.

Qualité de l'eau - Détermination spectrophotométrique de la teneur en chlorophylle a par extraction à l'éthanol pour la surveillance de routine de la qualité de l'eau

Le présent document décrit une méthode spectrophotométrique pour déterminer la teneur en chlorophylle a corrigée des phéopigments comme mesure de la quantité de phytoplancton dans tous les types d’eaux, y compris l’eau de mer. La limite de détermination est généralement de 2 µg/l à 5 µg/l et est calculée par chaque laboratoire individuellement. Elle peut atteindre 0,5 µg/l en utilisant 2 l d'échantillon (voire plus) et une cuve de 50 mm.
NOTE   Dans certains programmes de mesure tels que les études marines sur les données de séries chronologiques et le statut/la classification écologique, aucune correction des phéopigments n’est utilisée et l’acidification est omise, par exemple conformément aux recommandations d’OSPAR.

Kakovost vode - Spektrofotometrijsko določevanje klorofila-a po ekstrakciji z etanolom za rutinski monitoring kakovosti vode

General Information

• Status: Not Published
• Publication Date: 03-Jul-2024
• ICS: 13.060.70 - Examination of biological properties of water
• ICS: 71.040.50 - Physicochemical methods of analysis
• Technical Committee: CEN/TC 230 - Water analysis
• Drafting Committee: CEN/TC 230/WG 23 - Aquatic macrophytes and algae
• Current Stage: 5020 - Submission to Vote - Formal Approval
• Start Date: 14-Mar-2024
• Due Date: 12-Dec-2022
• Completion Date: 14-Mar-2024
• Ref Project: oSIST prEN 17899:2022 - Water quality - Spectrophotometric determination of chlorophyll-a content by ethanol extraction for the routine monitoring of water quality

Standards Content (Sample)

SLOVENSKI STANDARD
oSIST prEN 17899:2022
01-oktober-2022
Kakovost vode - Spektrofotometrijsko določevanje klorofila-a po ekstrakciji z
etanolom za rutinski monitoring kakovosti vode
Water quality - Spectrophotometric determination of chlorophyll-a content by ethanol
extraction for the routine monitoring of water quality
Wasserbeschaffenheit - Spektrophotometrische Bestimmung des Chlorophyll-a-Gehalts
durch Ethanolextraktion für das Routinemonitoring der Wasserqualität
Ta slovenski standard je istoveten z: prEN 17899
ICS:
13.060.70 Preiskava bioloških lastnosti Examination of biological
vode properties of water
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
oSIST prEN 17899:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

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oSIST prEN 17899:2022

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oSIST prEN 17899:2022


DRAFT
EUROPEAN STANDARD
prEN 17899
NORME EUROPÉENNE

EUROPÄISCHE NORM

September 2022
ICS 13.060.70; 71.040.50
English Version

Water quality - Spectrophotometric determination of
chlorophyll-a content by ethanol extraction for the routine
monitoring of water quality
 Wasserbeschaffenheit - Spektrophotometrische
Bestimmung des Gehalts an Chlorophyll-a
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 230.

If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.

This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.

CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.

Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.

Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.


EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION

EUROPÄISCHES KOMITEE FÜR NORMUNG

CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 17899:2022 E
worldwide for CEN national Members.

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oSIST prEN 17899:2022
prEN 17899:2022 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 6
5 Interferences . 6
6 Reagents . 6
7 Apparatus . 7
8 Procedure.
...