SIST EN ISO 24199:2022
(Main)Vapour products - Determination of nicotine in vapour product emissions - Gas chromatographic method (ISO 24199:2022)
Vapour products - Determination of nicotine in vapour product emissions - Gas chromatographic method (ISO 24199:2022)
This document specifies an analytical method to quantify nicotine of collected vapour product
emissions by gas chromatography.
Dampfprodukte - Bestimmung von Nikotin in Emissionen von Dampfprodukten - Gaschromatographisches Verfahren (ISO 24199:2022)
Dieses Dokument legt ein gaschromatographisches Analyseverfahren zur Quantifizierung von Nikotin in gesammelten Emissionen von Dampfprodukten fest.
Produits de vapotage - Détermination de la teneur en nicotine dans les émissions de produits de vapotage - Méthode par chromatographie en phase gazeuse (ISO 24199:2022)
Le présent document spécifie une méthode analytique destinée à quantifier par chromatographie en phase gazeuse le taux de nicotine dans les émissions de produit de vapotage collectées.
Hlapni proizvodi - Ugotavljanje deleža nikotina v emisijah hlapnih proizvodov - Metoda plinske kromatografije (ISO 24199:2022)
Ta dokument določa analitično metodo za ugotavljanje deleža nikotina v emisijah hlapnih proizvodov s plinsko kromatografijo.
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 24199:2022
01-september-2022
Hlapni proizvodi - Ugotavljanje deleža nikotina v emisijah hlapnih proizvodov -
Metoda plinske kromatografije (ISO 24199:2022)
Vapour products - Determination of nicotine in vapour product emissions - Gas
chromatographic method (ISO 24199:2022)
Dampfprodukte - Bestimmung von Nikotin in Emissionen von Dampfprodukten -
Gaschromatographisches Verfahren (ISO 24199:2022)
Produits de vapotage - Détermination de la teneur en nicotine dans les émissions de
produits de vapotage - Méthode par chromatographie en phase gazeuse (ISO
24199:2022)
Ta slovenski standard je istoveten z: EN ISO 24199:2022
ICS:
65.160 Tobak, tobačni izdelki in Tobacco, tobacco products
oprema and related equipment
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
SIST EN ISO 24199:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN ISO 24199:2022
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SIST EN ISO 24199:2022
EN ISO 24199
EUROPEAN STANDARD
NORME EUROPÉENNE
July 2022
EUROPÄISCHE NORM
ICS 65.160
English Version
Vapour products - Determination of nicotine in vapour
product emissions - Gas chromatographic method (ISO
24199:2022)
Produits de vapotage - Détermination de la teneur en Dampfprodukte - Bestimmung von Nikotin in
nicotine dans les émissions de produits de vapotage - Emissionen von Dampfprodukten -
Méthode par chromatographie en phase gazeuse (ISO Gaschromatographisches Verfahren (ISO 24199:2022)
24199:2022)
This European Standard was approved by CEN on 2 July 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 24199:2022 E
worldwide for CEN national Members.
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SIST EN ISO 24199:2022
EN ISO 24199:2022 (E)
Contents Page
European foreword . 3
2
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SIST EN ISO 24199:2022
EN ISO 24199:2022 (E)
European foreword
This document (EN ISO 24199:2022) has been prepared by Technical Committee ISO/TC 126 "Tobacco
and tobacco products" in collaboration with Technical Committee CEN/TC 437 “Electronic cigarettes
and e-liquids” the secretariat of which is held by AFNOR.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by January 2023, and conflicting national standards shall
be withdrawn at the latest by January 2023.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and the
United Kingdom.
Endorsement notice
The text of ISO 24199:2022 has been approved by CEN as EN ISO 24199:2022 without any modification.
3
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SIST EN ISO 24199:2022
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SIST EN ISO 24199:2022
INTERNATIONAL ISO
STANDARD 24199
First edition
2022-07
Vapour products — Determination of
nicotine in vapour product emissions
— Gas chromatographic method
Produits de vapotage — Détermination de la teneur en nicotine
dans les émissions de produits de vapotage — Méthode par
chromatographie en phase gazeuse
Reference number
ISO 24199:2022(E)
© ISO 2022
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SIST EN ISO 24199:2022
ISO 24199:2022(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
© ISO 2022 – All rights reserved
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SIST EN ISO 24199:2022
ISO 24199:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 3
7 Procedure .3
7.1 Preparation of test samples . 3
7.2 Glass fibre filter pads handling . 3
7.3 Aerosol collection and sample preparation . 3
7.4 Test portion . 4
7.5 Setting up the apparatus . 4
7.6 Calibration of the gas chromatograph . 5
7.7 Determination . 5
8 Expression of results . 5
9 Repeatability and reproducibility .6
9.1 General . 6
9.2 Results of an interlaboratory study (Study 1) . 6
9.3 Results of an interlaboratory study (Study 2) . 6
10 Test report . 7
Annex A (informative) Example chromatogram for nicotine analysis . 8
Bibliography . 9
iii
© ISO 2022 – All rights reserved
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SIST EN ISO 24199:2022
ISO 24199:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products,
Subcommittee SC 3, Vape and vapour products, in collaboration with the European Committee for
Standardization (CEN) Technical Committee CEN/TC 437, Electronic cigarettes and e-liquids, in
accordance with the Agreement on technical cooperation between ISO and CEN (Vienna Agreement).
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
© ISO 2022 – All rights reserved
---------------------- Page: 10 ----------------------
SIST EN ISO 24199:2022
ISO 24199:2022(E)
Introduction
In many countries, the regulation of vapour products requires reporting for nicotine compounds in
emissions. Therefore, there is a necessity to have an International Standard in place to get reliable/
comparable data on nicotine in electronic cigarette emissions.
This document was developed for the determination of nicotine in the aerosol from vapour products
utilizing gas chromatography coupled with a flame ionization detector. The experimental design
[1][2]
parameters used to collect the aerosolised vapour should be evaluated and documented for each
analysis.
[3]
The document is based on the CORESTA recommended method (CRM) 84 , which was written on the
[4]
basis of the results obtained in an interlaboratory study conducted in 2015 involving 18 laboratories
[5]
and an interlaboratory study conducted in 2019 involving 11 laboratories .
v
© ISO 2022 – All rights reserved
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SIST EN ISO 24199:2022
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SIST EN ISO 24199:2022
INTERNATIONAL STANDARD ISO 24199:2022(E)
Vapour products — Determination of nicotine in vapour
product emissions — Gas chromatographic method
1 Scope
This document specifies an analytical method to quantify nicotine of collected vapour product
emissions by gas chromatography.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 20768, Vapour products — Routine analytical vaping machine — Definitions and standard conditions
1)
ISO 24197:—, Vapour products — Determination of e-liquid vaporised mass and aerosol collected mass
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
vapour product
device intended for human use, which normally contains electronic components that vaporize a liquid
to generate an aerosol carried by the air drawn through the device by the user
[SOURCE: ISO 20768:2018, 3.1, modified – Note 1 to entry has been removed]
3.2
e-liquid
liquid or gel consumable which may or may not contain nicotine intended for transformation into an
aerosol and then inhaled with an electronic nicotine delivery device
[SOURCE: ISO 20714:2019, 3.1]
3.3
aerosol collected mass
ACM
mass of aerosol collected on a glass fibre filter pad resulting from the operation of a vapour product by
a routine analytical vaping machine after a defined number of puffs
Note 1 to entry: Routine analytical vaping machine is covered by ISO 20768.
1) Under preparation. Stage at the time of publication: ISO/DIS 24197:2022
1
© ISO 2022 – All rights reserved
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SIST EN ISO 24199:2022
ISO 24199:2022(E)
3.4
puff block
finite series of sequential puffs as defined by the user or by the test request
EXAMPLE Puff block 1: puffs 1 to 50, puff block 2: puffs 51 to 100, puff block 3: puffs 101 to 150.
4 Principle
The vapour product emissions shall be generated and collected as described in ISO 20768. The aerosol
collected mass is determined gravimetrically. The collected matter is then extracted with isopropanol
solution containing internal standard(s). The nico
...
SLOVENSKI STANDARD
oSIST prEN ISO 24199:2021
01-september-2021
Hlapni proizvodi - Ugotavljanje deleža nikotina v emisijah hlapnih proizvodov -
Metoda plinske kromatografije (ISO/DIS 24199:2021)
Vapour products - Determination of nicotine in vapour product emissions - Gas
chromatographic method (ISO/DIS 24199:2021)
Dampfprodukte - Bestimmung von Nikotin in Emissionen von Dampfprodukten -
Gaschromatographisches Verfahren (ISO/DIS 24199:2021)
Produits de vapotage - Détermination de la teneur en nicotine dans les émissions de
produits de vapotage - Méthode par chromatographie en phase gazeuse (ISO/DIS
24199:2021)
Ta slovenski standard je istoveten z: prEN ISO 24199
ICS:
65.160 Tobak, tobačni izdelki in Tobacco, tobacco products
oprema and related equipment
71.040.50 Fizikalnokemijske analitske Physicochemical methods of
metode analysis
oSIST prEN ISO 24199:2021 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
oSIST prEN ISO 24199:2021
---------------------- Page: 2 ----------------------
oSIST prEN ISO 24199:2021
DRAFT INTERNATIONAL STANDARD
ISO/DIS 24199
ISO/TC 126/SC 3 Secretariat: AFNOR
Voting begins on: Voting terminates on:
2021-07-05 2021-09-27
Vapour products — Determination of nicotine in vapour
product emissions — Gas chromatographic method
ICS: 65.160
THIS DOCUMENT IS A DRAFT CIRCULATED
This document is circulated as received from the committee secretariat.
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
ISO/CEN PARALLEL PROCESSING
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 24199:2021(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2021
---------------------- Page: 3 ----------------------
oSIST prEN ISO 24199:2021
ISO/DIS 24199:2021(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2021
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2021 – All rights reserved
---------------------- Page: 4 ----------------------
oSIST prEN ISO 24199:2021
ISO/DIS 24199:2021(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Reagents . 2
6 Apparatus . 3
7 Procedure. 3
7.1 Preparation of test samples . 3
7.2 Glass fibre filter pads handling . 3
7.3 Aerosol collection and sample preparation . 3
7.4 Test portion . 4
7.5 Setting up the apparatus. 4
7.6 Calibration of the gas chromatograph . 4
7.7 Determination . 5
8 Expression of results . 5
9 Repeatability and reproducibility . 5
9.1 Results of an interlaboratory study (Study 1) . 5
9.2 Results of an interlaboratory study (Study 2) . 6
10 Test report . 7
Annex A (informative) Example chromatogram for nicotine analysis . 8
Bibliography . 9
© ISO 2021 – All rights reserved iii
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oSIST prEN ISO 24199:2021
ISO/DIS 24199:2021(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www .iso .org/
iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products,
Subcommittee SC 3, vape and vapour products.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2021 – All rights reserved
---------------------- Page: 6 ----------------------
oSIST prEN ISO 24199:2021
ISO/DIS 24199:2021(E)
Introduction
In many countries regulation of vapour products require reporting for nicotine compounds in
emissions. Therefore, there is a necessity to have an International Standard in place to get reliable/
comparable data on nicotine in electronic cigarette emissions.
[9]
The method in this document is based upon the CORESTA recommended method (CRM) 84 , which
was written on the basis of the results obtained in an interlaboratory study conducted in 2015 involving
[12] [13]
18 laboratories and an interlaboratory study conducted in 2019 involving 11 laboratories .
This document has been developed to describe the procedures used to measure the amount of nicotine
in the aerosol from vapour products utilizing gas chromatography coupled with a flame ionization
[10][11]
detector. The experimental designing parameters used to collect the aerosolised vapour should
be evaluated and documented for each analysis.
© ISO 2021 – All rights reserved v
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oSIST prEN ISO 24199:2021
---------------------- Page: 8 ----------------------
oSIST prEN ISO 24199:2021
DRAFT INTERNATIONAL STANDARD ISO/DIS 24199:2021(E)
Vapour products — Determination of nicotine in vapour
product emissions — Gas chromatographic method
1 Scope
This document specifies an analytical method to quantify nicotine of collected vapour product
emissions by gas chromatography.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 20768, Vapour products — Routine analytical vaping machine — Definitions and standard conditions
ISO 24197, Vapour products — Determination of e-liquids vaporised mass and aerosol collected mass
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
vapour product
device intended for human use, which normally contains electronic components that vaporize a liquid
to generate an aerosol carried by the air drawn through the device by the user
[SOURCE: ISO 20768:2018, 3.1, modified – Note 1 to entry has been removed]
3.2
e-liquid
liquid or gel consumable which may or may not contain nicotine intended for transformation into an
aerosol and then inhaled with an electronic nicotine delivery device
[SOURCE: ISO 20714:2019, 3.1 modified – Reference to subsequent definition of electronic nicotine
delivery device has been removed]
3.3
aerosol collected mass
ACM
mass of aerosol collected on a glass fibre filter pad resulting from the operation of a vapour product by
a routine analytical vaping machine after a defined number of puffs
Note 1 to entry: Routine analytical vaping machine is covered by ISO 20768.
© ISO 2021 – All rights reserved 1
---------------------- Page: 9 ----------------------
oSIST prEN ISO 24199:2021
ISO/DIS 24199:2021(E)
3.4
puff block
finite series of sequential puffs as defined by the user or by the test request
EXAMPLE Puff block 1: puffs 1 to 50, puff block 2: puffs 51 to 100, puff block 3: puffs 101 to 150.
4 Principle
The vapour product emissions are generated and collected as described in ISO 20768. The aerosol
collected mass is determined gravimetrically. The collected matter is then extracted with isopropanol
solution containing internal standard(s). The nicotine content of an aliquot of the solution is determined
by capillary gas chromatography with flame ionization detection (GC-FID), and quantitated by internal
standard calibration. The nicotine content in the vapour product emissions is calculated. Results
are expressed as the weight of nicotine per puff, per aerosol collect mass (ACM), or per puff block as
warranted.
5 Reagents
Use only reagents of recognized analytical grade.
5.1 Carrier gas: helium (CAS 7440-59-7), nitrogen (CAS 7727-37-9), or hydrogen (CAS 1333-74-0) of
high purity.
5.2 Auxiliary gases: air and hydrogen (CAS 1333-74-0) of high purity for the flame ionization detector.
5.3 Isopropanol (CAS 67-63-0), minimum purity 99 %, used with internal standard(s) to prepare the
extraction solution.
5.4 Internal standards of high purity: quinaldine(CAS 91-63-4) or n-heptadecane (CAS 629-78-7) of
purity not less than 99 %.
n-octadecane (CAS 593-45-3) or other appropriate internal standards may be used after assessment
of their purity and determination that the internal standard does not co-elute with other components
in the sample extract. The peak area of the internal standard on samples should be monitored for
consistency. In cases where inconsistencies are found, analysis of a prepared sample solution without
the internal standard should be performed to confirm the absence of a peak in the extract eluting at the
same time as the internal standard.
...
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