SIST EN ISO 8655-1:2022
(Main)Piston-operated volumetric apparatus - Part 1: Terminology, general requirements and user recommendations (ISO 8655-1:2022)
Piston-operated volumetric apparatus - Part 1: Terminology, general requirements and user recommendations (ISO 8655-1:2022)
This document specifies general requirements for piston-operated volumetric apparatus (POVA). It
is applicable to pipettes, burettes, dilutors, dispensers and manually operated precision laboratory
syringes. It furthermore defines terms for the use of piston-operated volumetric apparatus and gives
user recommendations.
This document does not apply to medical products intended for use on humans, e.g. for medical syringes.
Volumenmessgeräte mit Hubkolben - Teil 1: Begriffe, allgemeine Anforderungen und Gebrauchsempfehlungen (ISO 8655-1:2022)
Dieser Teil von ISO 8655 legt die allgemeinen Anforderungen an Volumenmessgeräte mit HubkolbenN1) fest. Er gilt für Pipetten, Büretten, Dilutoren und Dispenser sowie manuell betätigte Präzisionslaborspritzen. Er definiert des Weiteren Begriffe für den Gebrauch von Kolbenhubgeräten und gibt Gebrauchsempfehlungen.
ISO 8655 gilt nicht für Medizinprodukte zur Anwendung am Menschen, z. B. für medizinische Spritzen.
ANMERKUNG Hinsichtlich der messtechnischen Anforderungen, der Fehlergrenzen sowie der Anforderungen an Kennzeichnung und Nutzerinformation siehe ISO 8655-2 für Pipetten, ISO 8655-3 für Büretten, ISO 8655-4 für Dilutoren, ISO 8655-5 für Dispenser und ISO 8655-9 für manuell betätigte Präzisionslaborspritzen. Das gravimetrische Referenzmessverfahren für die Volumenbestimmung ist in ISO 8655-6 angegeben. Das photometrische Referenzmessverfahren für die Volumenbestimmung ist in ISO 8655-8 angegeben. Alternative Messverfahren für die Volumenbestimmung werden in ISO 8655-7 beschrieben.
Appareils volumétriques à piston - Partie 1: Définitions, exigences générales et recommandations pour l'utilisateur (ISO 8655-1:2022)
Le présent document spécifie les exigences générales relatives aux appareils volumétriques à piston (AVAP). Elle est applicable aux pipettes, aux burettes, aux diluteurs, aux distributeurs et aux seringues de laboratoire haute précision pour utilisation manuelle. En outre, elle définit les conditions d’utilisation des appareils volumétriques à piston et donne des recommandations pour l’utilisateur.
Le présent document ne s’applique pas aux produits médicaux destinés à être utilisés sur les êtres humains, tels que les seringues médicales.
Volumetrične naprave, delujoče na bat - 1. del: Terminologija, splošne zahteve in priporočila za uporabnike (ISO 8655-1:2022)
Ta dokument določa splošne zahteve za volumetrične naprave, delujoče na bat (POVA). Ne uporablja se za pipete, birete, dilutorje, dozirnike in natančne laboratorijske brizge z ročnim upravljanjem. Opredeljuje tudi izraze za uporabo volumetričnih naprav, delujočih na bat, ter vsebuje priporočila za uporabnike. Ta dokument se ne uporablja za medicinske izdelke, namenjene uporabi pri ljudeh, npr. medicinske injekcijske brizge.
General Information
Relations
Standards Content (Sample)
SLOVENSKI STANDARD
SIST EN ISO 8655-1:2022
01-julij-2022
Nadomešča:
SIST EN ISO 8655-1:2002
SIST EN ISO 8655-1:2002/AC:2009
Volumetrične naprave, delujoče na bat - 1. del: Terminologija, splošne zahteve in
priporočila za uporabnike (ISO 8655-1:2022)
Piston-operated volumetric apparatus - Part 1: Terminology, general requirements and
user recommendations (ISO 8655-1:2022)
Volumenmessgeräte mit Hubkolben - Teil 1: Begriffe, allgemeine Anforderungen und
Gebrauchsempfehlungen (ISO 8655-1:2022)
Appareils volumétriques à piston - Partie 1: Définitions, exigences générales et
recommandations pour l'utilisateur (ISO 8655-1:2022)
Ta slovenski standard je istoveten z: EN ISO 8655-1:2022
ICS:
01.040.17 Meroslovje in merjenje. Metrology and measurement.
Fizikalni pojavi (Slovarji) Physical phenomena
(Vocabularies)
17.060 Merjenje prostornine, mase, Measurement of volume,
gostote, viskoznosti mass, density, viscosity
71.040.20 Laboratorijska posoda in Laboratory ware and related
aparati apparatus
SIST EN ISO 8655-1:2022 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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SIST EN ISO 8655-1:2022
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SIST EN ISO 8655-1:2022
EN ISO 8655-1
EUROPEAN STANDARD
NORME EUROPÉENNE
May 2022
EUROPÄISCHE NORM
ICS 01.040.17; 17.060 Supersedes EN ISO 8655-1:2002, EN ISO 8655-
1:2002/AC:2009
English Version
Piston-operated volumetric apparatus - Part 1:
Terminology, general requirements and user
recommendations (ISO 8655-1:2022)
Appareils volumétriques à piston - Partie 1: Volumenmessgeräte mit Hubkolben - Teil 1: Begriffe,
Définitions, exigences générales et recommandations allgemeine Anforderungen und
pour l'utilisateur (ISO 8655-1:2022) Gebrauchsempfehlungen (ISO 8655-1:2022)
This European Standard was approved by CEN on 13 February 2022.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this
European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN
member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by
translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management
Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and
United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2022 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN ISO 8655-1:2022 E
worldwide for CEN national Members.
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SIST EN ISO 8655-1:2022
EN ISO 8655-1:2022 (E)
Contents Page
European foreword . 3
2
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SIST EN ISO 8655-1:2022
EN ISO 8655-1:2022 (E)
European foreword
This document (EN ISO 8655-1:2022) has been prepared by Technical Committee ISO/TC 48
"Laboratory equipment" in collaboration with Technical Committee CEN/TC 332 “Laboratory
equipment” the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an
identical text or by endorsement, at the latest by November 2022, and conflicting national standards
shall be withdrawn at the latest by November 2022.
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
This document supersedes EN ISO 8655-1:2002.
Any feedback and questions on this document should be directed to the users’ national standards
body/national committee. A complete listing of these bodies can be found on the CEN website.
According to the CEN-CENELEC Internal Regulations, the national standards organizations of the
following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria,
Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland,
Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Republic of
North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the
United Kingdom.
Endorsement notice
The text of ISO 8655-1:2022 has been approved by CEN as EN ISO 8655-1:2022 without any
modification.
3
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SIST EN ISO 8655-1:2022
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SIST EN ISO 8655-1:2022
INTERNATIONAL ISO
STANDARD 8655-1
Second edition
2022-04
Piston-operated volumetric
apparatus —
Part 1:
Terminology, general requirements
and user recommendations
Appareils volumétriques à piston —
Partie 1: Définitions, exigences générales et recommandations pour
l'utilisateur
Reference number
ISO 8655-1:2022(E)
© ISO 2022
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SIST EN ISO 8655-1:2022
ISO 8655-1:2022(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2022
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
© ISO 2022 – All rights reserved
---------------------- Page: 8 ----------------------
SIST EN ISO 8655-1:2022
ISO 8655-1:2022(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated terms . 6
5 Types of piston-operated volumetric apparatus . 6
5.1 General . 6
5.2 Pipettes . 6
5.3 Burettes . 7
5.4 Dilutors . 7
5.5 Dispensers . 7
5.6 Syringes . 7
6 General requirements . 7
6.1 Frequency of calibrations and tests . 7
6.2 Reporting measurement errors . 7
6.3 Exchangeable Parts . 7
6.4 Metrological confirmation . 8
6.5 Routine testing . 8
6.6 Maintenance and repair . 8
6.7 Suitability to give statements on performance . 8
6.8 Determination of pass/fail status . 9
7 Product information . 9
7.1 User information . 9
7.2 Decontamination and sterilisation . 9
7.3 Chemical resistance . 9
8 Factors affecting choice of piston-operated volumetric apparatus .9
8.1 Interaction with liquids . 9
8.2 Use of air displacement pipettes . 10
9 Marking . .10
Bibliography .11
iii
© ISO 2022 – All rights reserved
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SIST EN ISO 8655-1:2022
ISO 8655-1:2022(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to
the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT), see
www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 48, Laboratory equipment, in collaboration
with the European Committee for Standardization (CEN) Technical Committee CEN/TC 332, Laboratory
equipment, in accordance with the Agreement on technical cooperation between ISO and CEN (Vienna
Agreement).
This second edition cancels and replaces the first edition (ISO 8655-1:2002), which has been technically
revised. It also incorporates the Technical Corrigendum ISO 8655-1:2002/Cor.1:2008.
The main changes are as follows:
— ISO 8655-7, ISO 8655-8, and ISO 8655-9 have been added as normative references;
— abbreviated terms have been introduced as Clause 4;
— terms and definitions have been revised and some new ones included;
— general requirements for frequency of calibration, reporting measurement errors, exchangeable
parts, metrological confirmation, routine testing, and maintenance and repair, suitability of
statements on performance and determination of pass/fail status have been added in Clause 6.
A list of all parts in the ISO 8655 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
© ISO 2022 – All rights reserved
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SIST EN ISO 8655-1:2022
ISO 8655-1:2022(E)
Introduction
The ISO 8655 series addresses the needs of:
— manufacturers, as a basis for quality control including, where appropriate, the issuance of
manufacturer’s declarations;
— calibration laboratories, test houses, users of the equipment and other bodies as a basis for
independent calibration, testing, verification and routine tests.
The tests specified in the ISO 8655 series are intended to be carried out by trained personnel.
v
© ISO 2022 – All rights reserved
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SIST EN ISO 8655-1:2022
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SIST EN ISO 8655-1:2022
INTERNATIONAL STANDARD ISO 8655-1:2022(E)
Piston-operated volumetric apparatus —
Part 1:
Terminology, general requirements and user
recommendations
1 Scope
This document specifies general requirements for piston-operated volumetric apparatus (POVA). It
is applicable to pipettes, burettes, dilutors, dispensers and manually operated precision laboratory
syringes. It furthermore defines terms for the use of piston-operated volumetric apparatus and gives
user recommendations.
This document does not apply to medical products intended for use on humans, e.g. for medical syringes.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 8655-2, Piston-operated volumetric apparatus — Part 2: Pipettes
ISO 8655-3, Piston-operated volumetric apparatus — Part 3: Burettes
ISO 8655-4, Piston-operated volumetric apparatus — Part 4: Dilutors
ISO 8655-5, Piston-operated volumetric apparatus — Part 5: Dispensers
ISO 8655-6, Piston-operated volumetric apparatus — Part 6: Gravimetric reference measurement
procedure for the determination of volume
ISO 8655-7, Piston-operated volumetric apparatus — Part 7: Alternative measurement procedures for the
determination of volume
ISO 8655-8, Piston-operated volumetric apparatus — Part 8: Photometric reference measurement
procedure for the determination of volume
ISO 8655-9, Piston-operated volumetric apparatus — Part 9: Manually operated precision laboratory
syringes
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
1
© ISO 2022 – All rights reserved
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SIST EN ISO 8655-1:2022
ISO 8655-1:2022(E)
3.1
adjustment
〈POVA〉 set of operations carried out so that the delivered volume more closely corresponds to the
nominal or selected volume
Note 1 to entry: Information about the correct adjustment method can be found in the manufacturer’s instruction
manual. Some devices have more than one adjustment method. Some have none at all.
3.2
balance
〈POVA〉 device used to determine the mass of a delivered volume
3.3
bandpass filter
optical filter used to selectively transmit a band of wavelengths from the total spectrum while rejecting
shorter and longer wavelengths than those within the transmission band
3.4
calibration
〈POVA〉 set of operations that establish the relationship between the delivered volume and the
corresponding selected volume of the apparatus with associated measurement uncertainties
Note 1 to entry: Calibration requires no operation which permanently modifies the apparatus and does not
require adjustment of the device.
3.5
coefficient of variation
random error expressed as a percentage of the mean delivered volume
3.6
correction
mathematical compensation of a systematic effect
Note 1 to entry: In the context of this document, a systematic error can be mathematically compensated by
subtracting it from the selected volume.
Note 2 to entry: The correction is applied when the user is setting the indication to deliver the corrected intended
volume.
3.7
chromophore
compound which absorbs photons of a specific energy
3.8
dead air volume
headspace
captive air volume
air cushion
〈air displacement pipettes〉 air volume between the piston and the surface of the liquid in the tip
3.9
dead volume
〈POVA〉 amount of liquid that does not belong to the delivered volume and which is contained during
operation in aspiration or expelling tubes, valves and within the cylinder
3.10
evaporation trap
accessory used in a balance that minimizes evaporation of test liquid from the weighing vessel
3.11
immersion depth
〈piston pipette〉 depth of the tip orifice of the piston pipette below the liquid surface
2
© ISO 2022 – All rights reserved
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SIST EN ISO 8655-1:2022
ISO 8655-1:2022(E)
3.12
liquid handling process tolerance
user-specified tolerance defining the maximum acceptable uncertainty in use of the delivered volume
Note 1 to entry: A POVA is fit for its intended purpose if its uncertainty in use is smaller than or equal to the liquid
handling process tolerance.
Note 2 to entry: The liquid handling process tolerance is typically expressed as a percentage of the nominal or
selected volume.
3.13
maximum permis
...
SLOVENSKI STANDARD
oSIST prEN ISO 8655-1:2020
01-oktober-2020
Volumetrične naprave, delujoče na bat - 1. del: Terminologija, splošne zahteve in
priporočila za uporabnike (ISO/DIS 8655-1:2020)
Piston-operated volumetric apparatus - Part 1: Terminology, general requirements and
user recommendations (ISO/DIS 8655-1:2020)
Volumenmessgeräte mit Hubkolben - Teil 1: Begriffe, allgemeine Anforderungen und
Gebrauchsempfehlungen (ISO/DIS 8655-1:2020)
Appareils volumétriques à piston - Partie 1: Définitions, exigences générales et
recommandations pour l'utilisateur (ISO/DIS 8655-1:2020)
Ta slovenski standard je istoveten z: prEN ISO 8655-1
ICS:
01.040.17 Meroslovje in merjenje. Metrology and measurement.
Fizikalni pojavi (Slovarji) Physical phenomena
(Vocabularies)
17.060 Merjenje prostornine, mase, Measurement of volume,
gostote, viskoznosti mass, density, viscosity
71.040.20 Laboratorijska posoda in Laboratory ware and related
aparati apparatus
oSIST prEN ISO 8655-1:2020 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
---------------------- Page: 1 ----------------------
oSIST prEN ISO 8655-1:2020
---------------------- Page: 2 ----------------------
oSIST prEN ISO 8655-1:2020
DRAFT INTERNATIONAL STANDARD
ISO/DIS 8655-1
ISO/TC 48 Secretariat: DIN
Voting begins on: Voting terminates on:
2020-07-29 2020-10-21
Piston-operated volumetric apparatus —
Part 1:
Terminology, general requirements and user
recommendations
Appareils volumétriques à piston —
Partie 1: Définitions, exigences générales et recommandations pour l'utilisateur
ICS: 17.060; 01.040.17
THIS DOCUMENT IS A DRAFT CIRCULATED
This document is circulated as received from the committee secretariat.
FOR COMMENT AND APPROVAL. IT IS
THEREFORE SUBJECT TO CHANGE AND MAY
NOT BE REFERRED TO AS AN INTERNATIONAL
STANDARD UNTIL PUBLISHED AS SUCH.
IN ADDITION TO THEIR EVALUATION AS
ISO/CEN PARALLEL PROCESSING
BEING ACCEPTABLE FOR INDUSTRIAL,
TECHNOLOGICAL, COMMERCIAL AND
USER PURPOSES, DRAFT INTERNATIONAL
STANDARDS MAY ON OCCASION HAVE TO
BE CONSIDERED IN THE LIGHT OF THEIR
POTENTIAL TO BECOME STANDARDS TO
WHICH REFERENCE MAY BE MADE IN
Reference number
NATIONAL REGULATIONS.
ISO/DIS 8655-1:2020(E)
RECIPIENTS OF THIS DRAFT ARE INVITED
TO SUBMIT, WITH THEIR COMMENTS,
NOTIFICATION OF ANY RELEVANT PATENT
RIGHTS OF WHICH THEY ARE AWARE AND TO
©
PROVIDE SUPPORTING DOCUMENTATION. ISO 2020
---------------------- Page: 3 ----------------------
oSIST prEN ISO 8655-1:2020
ISO/DIS 8655-1:2020(E)
COPYRIGHT PROTECTED DOCUMENT
© ISO 2020
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address
below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii © ISO 2020 – All rights reserved
---------------------- Page: 4 ----------------------
oSIST prEN ISO 8655-1:2020
ISO/DIS 8655-1:2020(E)
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Abbreviated Terms . 7
5 Types of piston-operated volumetric apparatus . 8
5.1 General . 8
5.2 Pipettes . 8
5.3 Burettes . 8
5.4 Dilutors . 8
5.5 Dispensers . 8
5.6 Syringes . 8
6 General requirements . 8
6.1 Reference measurement procedures . 8
6.2 Suitability to give statements on performance . 9
6.3 Reporting measurement errors . 9
6.4 Test frequency . 9
6.5 Exchangeable Parts . 9
7 Routine testing . 9
8 Determination of pass/fail status .10
9 Product information .10
9.1 User information .10
9.2 Decontamination .10
9.3 Chemical resistance .10
10 Factors affecting choice of piston-operated volumetric apparatus .11
10.1 Interaction with liquids .11
10.2 Use of air displacement pipettes .11
11 Marking .11
Annex A (informative) List of equivalent terms .12
Bibliography .13
© ISO 2020 – All rights reserved iii
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oSIST prEN ISO 8655-1:2020
ISO/DIS 8655-1:2020(E)
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out
through ISO technical committees. Each member body interested in a subject for which a technical
committee has been established has the right to be represented on that committee. International
organizations, governmental and non-governmental, in liaison with ISO, also take part in the work.
ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are
described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of
patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of
any patent rights identified during the development of the document will be in the Introduction and/or
on the ISO list of patent declarations received (see www .iso .org/ patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and
expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following
URL: www .iso .org/ iso/ foreword .html.
This document was prepared by Technical Committee ISO/TC 48, Laboratory Equipment, Working
Group WG 4, Piston-operated instruments.
This second edition cancels and replaces the first edition (ISO 8655‐1:2002 and ISO 8655‐1:2002/Cor
1:2008), which has been technically revised.
The main changes compared to the previous edition are as follows:
— ISO 8655-7, ISO 8655-8, and ISO 8655-9 have been added as a normative references;
— Abbreviated terms have been introduced as Clause 4;
— Terms and definitions have been revised;
— General requirements for measurement capability and reference measurement have been added.
A list of all parts in the ISO 8655 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/ members .html.
iv © ISO 2020 – All rights reserved
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oSIST prEN ISO 8655-1:2020
ISO/DIS 8655-1:2020(E)
Introduction
ISO 8655 addresses the needs of:
— manufacturers, as a basis for quality control including, where appropriate, the issuance of
manufacturers' declarations;
— calibration laboratories, test houses, users of the equipment and other bodies as a basis for
independent calibration, certification and routine checking.
The tests specified in the ISO 8655 series are intended to be carried out by trained personnel.
© ISO 2020 – All rights reserved v
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oSIST prEN ISO 8655-1:2020
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oSIST prEN ISO 8655-1:2020
DRAFT INTERNATIONAL STANDARD ISO/DIS 8655-1:2020(E)
Piston-operated volumetric apparatus —
Part 1:
Terminology, general requirements and user
recommendations
1 Scope
This part of ISO 8655 specifies general requirements for piston‐operated volumetric apparatus (POVA).
It is applicable to pipettes, burettes, dilutors, dispensers and manually operated precision laboratory
syringes. It furthermore defines terms for the use of piston‐operated volumetric apparatus and gives
user recommendations.
ISO 8655 is not applicable to medical products intended for use on humans, e.g. for medical syringes.
NOTE For the metrological requirements, maximum permissible errors, requirements for marking and
information to be provided for users of piston-operated volumetric apparatus, see ISO 8655-2 for pipettes,
see ISO 8655-3 for burettes, see ISO 8655-4 for dilutors, see ISO 8655-5 for dispensers, and see ISO 8655-9 for
manually operated precision laboratory syringes. The gravimetric reference measurement procedure for the
determination of volume is given in ISO 8655-6. The photometric reference measurement procedure for the
determination of volume is given in ISO 8655-8. Alternative measurement procedures for the determination of
volume are described in ISO 8655-7.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content
constitutes requirements of this document. For dated references, only the edition cited applies. For
undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 8655-2:2020, Piston-operated volumetric apparatus — Part 2: Pipettes
ISO 8655-3:2020, Piston-operated volumetric apparatus — Part 3: Burettes
ISO 8655-4:2020, Piston-operated volumetric apparatus — Part 4: Dilutors
ISO 8655-5:2020, Piston-operated volumetric apparatus — Part 5: Dispensers
ISO 8655-6:2020, Piston-operated volumetric apparatus — Part 6: Gravimetric reference measurement
procedure for the determination of volume
ISO 8655-7:2020, Piston-operated volumetric apparatus — Part 7: Alternative measurement procedures
for the determination of volume
ISO 8655-8:2020, Piston-operated volumetric apparatus — Part 8: Photometric reference measurement
procedure for the determination of volume
ISO 8655-9:2020, Piston-operated volumetric apparatus — Part 9: Manually operated precision laboratory
syringes
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
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oSIST prEN ISO 8655-1:2020
ISO/DIS 8655-1:2020(E)
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at http:// www .electropedia .org/
3.1
absorbance drift
change in measured absorbance at a specific wavelength over time due to photometer characteristics
3.2
accuracy of measurement
〈piston‐operated volumetric apparatus〉 closeness of agreement between delivered volume and the
nominal or selected volume
Note 1 to entry: The concept ‘accuracy’ is not given a numerical value. Accuracy is a combination of trueness and
precision and can be assessed with both the systematic error and the random error and will be affected by both.
Note 2 to entry: The term ‘accuracy’ is not the same as ‘trueness”.
Note 3 to entry: The measurement uncertainty of a single delivered volume is used to quantitatively express the
accuracy of a measurement.
[SOURCE: ISO/IEC Guide 99: 2007, 2.13, modified for the purpose of the POVA standard]
3.3
adjustment
〈piston‐operated volumetric apparatus〉 set of operations carried out so that the delivered volume more
closely corresponds to the nominal or selected volume
Note 1 to entry: Information about the correct adjustment method can be found in the manufacturer’s instruction
manual. Some devices have more than one adjustment method.
[SOURCE: ISO/IEC Guide 99: 2007, 3.11, modified for the purpose of the POVA standard]
3.4
balance
〈piston‐operated volumetric apparatus〉 device to determine the mass of a delivered volume
Note 1 to entry: An analytical balance is a balance that has a readability of 0.1 mg or smaller.
Note 2 to entry: The readability is the smallest difference in mass that can be read on the balance.
3.5
bandpass filter
optical filter used to selectively transmit a band of wavelengths from the total spectrum while rejecting
shorter and longer wavelengths than those within the transmission band
3.6
baseline drift
photometric drift
photometric stability
change in the baseline absorbance at a specific wavelength over time
Note 1 to entry: Temperature fluctuations can influence the photometric stability.
3.7
baseline flatness
measure of the photometer’s ability to compensate for inherent optical and electronic noise across the
wavelength range of interest without any sample in the sample compartment
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oSIST prEN ISO 8655-1:2020
ISO/DIS 8655-1:2020(E)
3.8
calibration
〈piston‐operated volumetric apparatus〉 set of operations that establish the relationship between
the delivered volume and the corresponding nominal or selected volume of the apparatus with
measurement uncertainties
Note 1 to entry: Calibration requires no operation which permanently modifies the apparatus and does not
require adjustment of the device.
[SOURCE: ISO/IEC Guide 99: 2007, 2.39, modified for the purpose of the POVA standard]
3.9
coefficient of variation
the random error expressed as a percentage of the mean delivered volume
3.10
correction
mathematical compensation of a systematic effect
Note 1 to entry: In the context of this standard, a systematic error can be mathematically compensated by
subtracting the systematic error from the measured value.
Note 2 to entry: The correction is applied when the user is setting the indication in order to deliver the corrected
intended volume.
[SOURCE: ISO/IEC Guide 99: 2007, 2.53, modified for the purpose of the POVA standard]
3.11
chromophore
compound which absorbs photons of a specific energy
3.12
dead air volume
headspace
captive air volume
air cushion
〈air displacement pipettes〉 air volume between the piston and the surface of the liquid in the tip
3.13
dead liquid volume
〈piston‐operated volumetric apparatus〉 amount of liquid which does not belong to the delivered volume
and which is contained during operation in aspiration or expelling tubes, valves and within the cylinder
3.14
evaporation trap
accessory of a balance that minimizes evaporation of test liquid in the weighing vessel
3.15
immersion depth
〈piston pipette〉 depth of the tip orifice of the piston pipette below the liquid surface
3.16
liquid handling process tolerance
user‐specified tolerance defining the maximum acceptable uncertainty in use of the delivered volume
Note 1 to entry: A POVA is fit for its intended purpose if its uncertainty in use is smaller than or equal to the liquid
handling process tolerance.
Note 2 to entry: The liquid handling process tolerance is typically expressed as a percentage of the nominal or
selected volume.
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oSIST prEN ISO 8655-1:2020
ISO/DIS 8655-1:2020(E)
3.17
maximum permissible systematic error
permitted extreme value for the deviation of the mean delivered volume from the nominal volume (3.27)
or selected volume (3.34)
Note 1 to entry: The maximum permissible systematic errors of piston‐operated volumetric apparatus are
specified in ISO 8655‐2 to ISO 8655‐5, and ISO 8655‐9.
[SOURCE: ISO/IEC Guide 99:2007, 4.26, modified for the purpose of the POVA standard]
3.18
maximum permissible random error
permitted extreme value for the variation of the delivered volumes around the mean of the
delivered volumes
Note 1 to entry: The maximum permissible random errors of piston‐operated volumetric apparatus are specified
in ISO 8655-2 to ISO 8655-5 and ISO 8655-9.
[SOURCE: ISO/IEC Guide 99:2007, 4.26, modified for the purpose of the POVA standard]
3.19
mean delivered volume
arithmetic mean of multiple replicate delivered volumes obtained by the measurement method
3.20
measurement
process of experimentally obtaining one or more quantity values that can be reasonably attributed to a
quantity
[SOURCE: ISO/IEC Guide 99:2007, 2.1]
3.21
measurement repeatability
measurement precision under a set of repeatability conditions of measurement
[SOURCE: ISO/IEC Guide 99:2007, 2.21]
3.22
measurement uncertainty
〈volume delivered by a piston‐operated apparatus〉 non‐negative parameter, associated with the
delivered volume, that characterizes the dispersion of the volumes that could reasonably be attributed
to the delivered volume based on the information used
Note 1 to entry: There are two distinct types of measureme
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