prEN 18057
(Main)Food authenticity - Quantitation of roe deer DNA relative to mammalian DNA in meat and meat products
Food authenticity - Quantitation of roe deer DNA relative to mammalian DNA in meat and meat products
This document specifies a real-time PCR procedure for the quantification of the amount of roe deer DNA relative to total mammalian and poultry DNA in meat and meat products.
The results of this assay for roe deer are expressed in terms of roe deer (Capreolus capreolus) haploid genome copy numbers relative to mammalian total haploid genome copy numbers. The content of roe deer can also be expressed as a percentage by mass using gravimetrically prepared calibration material from meat mixtures or model samples.
The method has been previously validated in a collaborative trial and applied to DNA extracted from samples that consist of raw roe deer meat in a raw pig meat background as well as raw and boiled sausages.
The limit of detection of the roe deer PCR has been determined experimentally to be at least 5 target gene copies or 0,03 % roe deer.
The compliance assessment process is not part of this document.
Lebensmittelauthentizität - Quantifizierung von Reh-DNA im Verhältnis zu Säugetier-DNA in Fleisch und Fleischprodukten
Authenticité des aliments - Quantification de l'ADN de chevreuil par rapport à l'ADN de mammifère dans la viande et les produits carnés
Pristnost živil - Kvantitativno določanje DNK srnjadi glede na DNK sesalcev v mesu in mesnih izdelkih
General Information
Standards Content (Sample)
SLOVENSKI STANDARD
oSIST prEN 18057:2024
01-april-2024
Pristnost živil - Kvantitativno določanje DNK srnjadi glede na DNK sesalcev v
mesu in mesnih izdelkih
Food authenticity - Quantitation of roe deer DNA relative to mammalian DNA in meat and
meat products
Lebensmittelauthentizität - Quantifizierung von Reh-DNA im Verhältnis zu Säugetier-
DNA in Fleisch und Fleischprodukten
Authenticité des aliments - Quantification de l'ADN de chevreuil par rapport à l'ADN de
mammifère dans la viande et les produits carnés
Ta slovenski standard je istoveten z: prEN 18057
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.120.10 Meso in mesni proizvodi Meat and meat products
oSIST prEN 18057:2024 en,fr,de
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.
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oSIST prEN 18057:2024
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oSIST prEN 18057:2024
DRAFT
EUROPEAN STANDARD
prEN 18057
NORME EUROPÉENNE
EUROPÄISCHE NORM
February 2024
ICS 67.120.10; 07.100.30
English Version
Food authenticity - Quantitation of roe deer DNA relative
to mammalian DNA in meat and meat products
Authenticité des aliments - Quantification de l'ADN de Lebensmittelauthentizität - Quantifizierung von Reh-
chevreuil par rapport à l'ADN de mammifère dans la DNA im Verhältnis zu Säugetier-DNA in Fleisch und
viande et les produits carnés Fleischprodukten
This draft European Standard is submitted to CEN members for enquiry. It has been drawn up by the Technical Committee
CEN/TC 460.
If this draft becomes a European Standard, CEN members are bound to comply with the CEN/CENELEC Internal Regulations
which stipulate the conditions for giving this European Standard the status of a national standard without any alteration.
This draft European Standard was established by CEN in three official versions (English, French, German). A version in any other
language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC
Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,
Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway,
Poland, Portugal, Republic of North Macedonia, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Türkiye and
United Kingdom.
Recipients of this draft are invited to submit, with their comments, notification of any relevant patent rights of which they are
aware and to provide supporting documentation.
Warning : This document is not a European Standard. It is distributed for review and comments. It is subject to change without
notice and shall not be referred to as a European Standard.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
CEN-CENELEC Management Centre: Rue de la Science 23, B-1040 Brussels
© 2024 CEN All rights of exploitation in any form and by any means reserved Ref. No. prEN 18057:2024 E
worldwide for CEN national Members.
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oSIST prEN 18057:2024
prEN 18057:2024 (E)
Contents Page
European foreword . 3
Introduction . 4
1 Scope . 5
2 Normative references . 5
3 Terms and definitions . 5
4 Principle . 5
5 Reagents and materials . 6
6 Apparatus . 7
7 Procedure . 7
7.1 Preparation of the test portion/sample . 7
7.2 Preparation of DNA extracts .
...
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